GE17-5158-01
HiTrap® Q XL
Cytiva 17-5158-01, pack of 5 × 1 mL
About This Item
Recommended Products
ligand
quaternary amine
description
Ion Exchanger Type (value)
form
prepacked column
crosslinking
cross-linked
packaging
pack of 5 × 1 mL
manufacturer/tradename
Cytiva 17-5158-01
storage condition
(20% Ethanol)
parameter
-70 psi max. pressure (max flow rate = 20 ml/min)
technique(s)
liquid chromatography (LC): suitable
bed size
7 mm × 25 mm
bed volume
1 mL
column I.D.
7 mm
matrix
6% cross-linked agarose with dextran surface extenders
matrix active group
Q functional group, ammonium group
particle size
45-165 μm
average diameter
90 μm
cleaning
2-14
working range
2-12
capacity
binding capacity(>= 160 mg albumin / mL)
ion exchange capacity(0.18-0.26 mmol Cl / mL)
suitability
suitable for bioprocess medium
General description
Application
Features and Benefits
- High binding capacities with high fLow rates.
- Convenient and affordable for fast, easy separations either alone or connected in series.
- Designed for use with syringe, peristaltic pump, or chromatography system such as ÄKTA® design.
Storage and Stability
Analysis Note
Legal Information
related product
Signal Word
Warning
Hazard Statements
Precautionary Statements
Storage Class Code
3 - Flammable liquids
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Articles
This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.
This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.
This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.
Protocols
This page covers how and when to use Sepharose XL media for purification of proteins.
This page discusses how to do a purification with SOURCE™ media from GE Healthcare
This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.
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