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GE17-5112-01

HiTrap® Streptavidin High Performance

Cytiva 17-5112-01, pack of 5 × 1 mL

Synonym(s):

HiTrap Streptavidin, Streptavidin High Performance

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About This Item

UNSPSC Code:
23151816
NACRES:
NA.56

packaging

pack of 5 × 1 mL

manufacturer/tradename

Cytiva 17-5112-01

storage condition

(20% Ethanol)
 (20% Ethanol, 0.1 M Potassium Phosphate buffer, pH 8)

parameter

42 psi

bed size

7 mm × 25 mm

bed volume

1 mL

column I.D.

7 mm

matrix

highly cross-linked 6% agarose

avg. part. size

34 μm

cleaning

2-10.5

working range

4-9

capacity

binding capacity(>300 nmol biotin/ml medium 6 mg biotinylated BSA/ml medium)

storage temp.

2-8°C

General description

HiTrap® Streptavidin HP is a column prepacked with Streptavidin Sepharose™ High Performance for purification of biotinylated proteins and biomolecules.

Application

The prepacked Streptavidin Sepharose™ High Performance medium ensures reproducibility, high performance, and excellent physical and chemical stability. Streptavidin Sepharose™ High Performance is also available in lab packs, using the same purified streptavidin isolated from Streptomyces avidinii bound to Sepharose™ High Performance.

Features and Benefits

  • Convenient, fast, reliable purification of biotinylated compounds.
  • The strong interactions of biotin and streptavidin, as well as the somewhat weaker interactions of 2-iminobiotin and streptavidin are utilized.
  • The column can be operated with a syringe, peristaltic pump, or liquid chromatography system such as ÄKTA® design or FPLC™ System.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

FPLC is a trademark of Cytiva
HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva
ÄKTA is a registered trademark of Cytiva

related product

Product No.
Description
Pricing

Pictograms

Flame
GHS02

Signal Word

Warning

Hazard Statements

H226

Storage Class Code

3 - Flammable liquids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

Affinity Chromatography Troubleshooting

This page shows how to solve practical problems that may occur when running an affinity chromatography column.

Protocols

Purification or Removal of Biotin and Biotinylated Substances

This page shows how to purify or remove biotin and biotinylated substances with Streptavidin Sepharose High Performance from GE Healthcare.

Converting From Linear Fow (cm/hour) to Volumetric Flow Rates (mL/min) and Vice Versa

This page shows how to convert between linear flow and volumetric flow rates in affinity chromatography.

Column Packing and Preparation Protocol for Affinity Chromatography

This page shows how to perform column packing and preparation for affinity chromatography when using Tricorn™ or XK columns available from GE Healthcare.

Sample Preparation for Affinity Chromatography

This page shows how to prepare samples for purification with affinity chromatography.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service