GE17-0906-01
NHS Act Sepharose™ 4 Fast Flow
Cytiva 17-0906-01, pack of 25 mL
Synonym(s):
NHS-Activated Sepharose FF, pre-activated agarose matrix
About This Item
Recommended Products
ligand
N-hydroxysuccinimide activated groups
packaging
pack of 25 mL
manufacturer/tradename
Cytiva 17-0906-01
storage condition
(100% Isopropanol)
matrix
4% cross-linked agarose
particle size
45-165 μm
average diameter
90 μm
cleaning in place
3-13(Ligand dependent)
ligand dependent
3-13
working range
3-12(3 (ligand dependent)-12 (ligand dependent))
suitability
suitable for bioprocess medium
storage temp.
2-8°C
General description
NHS-activated Sepharose™ 4 Fast Flow is composed of cross-linked 4% agarose beads, NHS activated for coupling amino-containing proteins and peptides in process-scale applications. NHS (N-hydroxysuccinimide) coupling forms a chemically stable amide bond with ligands containing primary amino groups.
This resin provides a spacer arm to the coupled ligand and is therefore particularly suitable for immobilising smaller protein and peptide ligands.
NHS-activated Sepharose™ 4 Fast Flow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development.
As member of the BioProcess media range, NHS-activated Sepharose™ 4 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.
Features and Benefits
- NHS pre-activated medium for coupling of small amino-containing proteins and peptides in process-scale applications
- High level of pre-activation gives a high degree of substitution of the selected ligand
- NHS coupling method results in chemically stable ligand attachment
- BioProcess medium supported for industrial applications
Storage and Stability
Analysis Note
Legal Information
Signal Word
Danger
Hazard Statements
Precautionary Statements
Storage Class Code
3 - Flammable liquids
Certificates of Analysis (COA)
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Articles
This page describes efficient column packing and preparation for affinity chromatography of antibodies.
This page shows how to perform sample desalting and buffer exchange for affinity chromatography of antibodies.
Protocols
This page shows coupling through the primary amine of a ligand with a NHS-activated Sepharose High Performance, NHS-activated Sepharose 4 Fast Flow and CNBr-activated Sepharose from Cytiva.
Column packing and preparation for Affinity Chromatography with specific groups of biomolecules.
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