GE17-0998-01
Heparin Sepharose™ 6 Fast Flow
Cytiva 17-0998-01, pack of 50 mL
About This Item
Recommended Products
ligand
porcine heparin
shelf life
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging
pack of 50 mL
manufacturer/tradename
Cytiva 17-0998-01
matrix
6% cross-linked agarose
particle size
45-165 μm
average diameter
90 μm
cleaning in place
4-13
working range
4-12
suitability
suitable for bioprocess medium
Related Categories
General description
Heparin Sepharose™ 6 Fast Flow is a member of the Cytiva range of affinity chromatography media for capture and intermediate purification.
Heparin Sepharose™ 6 Fast Flow is composed of cross-linked 6% agarose beads modified sodium heparin. Immobilization is performed using chemically stable and oriented coupling giving high binding capacity. The chemical stability of the media is solely dictated by the heparin ligand.
This medium allows for fast and reliable separations of biomolecules with an affinity for heparin, including antithrombin III, coagulation factors and other plasma proteins. Additional applications include DNA binding proteins, lipoproteins, protein synthesis factors, enzymes that act on nucleic acids, and steroid receptors.
Heparin Sepharose™ 6 Fast Flow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development.
As member of the BioProcess media range, Heparin Sepharose™ 6 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.
Features and Benefits
- Industrial purification of antithrombin III, coagulation factors and other plasma proteins
- Coupling chemistry gives chemically-stable ligand attachment. Stability of medium solely dictated by heparin ligand.
- BioProcess medium supported for industrial applications and well-established in approved processes
Storage and Stability
Analysis Note
Legal Information
Signal Word
Warning
Hazard Statements
Precautionary Statements
Storage Class Code
3 - Flammable liquids
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Articles
This page shows how to remove serine proteases, e.g. thrombin and trypsin, and zymogens from recombinant products using HiTrap Benzamidine FF and Benzamidine Sepharose 4 Fast Flow from GE Healthcare.
This page shows how to solve practical problems that may occur when running an affinity chromatography column.
Protocols
DNA binding proteins form an extremely diverse class of proteins sharing a single characteristic, their ability to bind to DNA. Functionally the group can be divided into those responsible for the replication and orientation of the DNA such as histones, nucleosomes and replicases and those involved in transcription such as RNA/DNA polymerases, transcriptional activators and repressors and restriction enzymes.
How to perform a separation with GST fusion proteins which are affinity chromatography tags used in GST MicroSpin Purifcation Module, GSTrap FF, GSTPrep FF 16/10, Glutathione Sepharose 4 Fast Flow and Glutathione Sepharose 4B products from GE Healthcare.
This page shows how to perform column packing and preparation for affinity chromatography when using Tricorn™ or XK columns available from GE Healthcare.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service