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G0774

Sigma-Aldrich

Glycerokinase from Bacillus stearothermophilus

buffered aqueous solution, ≥75 units/mg protein (biuret)

Synonym(s):

ATP:glycerol 3-phosphotransferase, Glycerol Kinase

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About This Item

CAS Number:
9030-66-4
Enzyme Commission number:
2.7.1.30 (BRENDA, IUBMB)
EC Number:
232-862-0
MDL number:
MFCD00131205
UNSPSC Code:
12352204
eCl@ss:
32160410
NACRES:
NA.54

biological source

Bacillus sp. (Bacillus steaarothermophilus)

Assay

≥5.0 mg protein/mL (biuret)

form

buffered aqueous solution

specific activity

≥75 units/mg protein (biuret)

storage condition

dry at room temperature

color

beige

application(s)

life science and biopharma

storage temp.

2-8°C

Gene Information

Bacillus sp. ... glpK(89613566)

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General description

Research area: Cell Signaling

Glycerol kinase is encoded by the GK gene on chromosome Xp21.2. Glycerol kinase is predominantly active in the liver.

Application

Glycerokinase from Bacillus stearothermophilus has been used in steady-state kinetics studies.

Biochem/physiol Actions

Glycerol kinase catalyzes tge MgATP-dependent phosphorylation of glycerol to produce sn-glycerol-3-phosphate and is the rate limiting enzyme in the utilization of glycerol. It is also subject to feedback regulation by fructose-1,6-bisphosphate.Oxidized glycerol-3-phosphate results in dihydroxyacetone phosphate which enters either glycolysis or gluconeogenesis. Elevated glycerol is observed in glycerol kinase deficiency leading to pseudohypertriglyceridemia.

Unit Definition

One unit will convert 1.0 μmole of glycerol and ATP to L-α-glycerophosphate and ADP per min at pH 9.8 at 25 °C in a coupled system with PK/LDH.

Physical form

Stabilized solution in Tris buffer, pH 7.3

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Purification and properties of glycerol kinase from Escherichia coli.
S I Hayashi et al.
The Journal of biological chemistry, 242(5), 1030-1035 (1967-03-10)
N Zwaig et al.
Journal of bacteriology, 102(3), 753-759 (1970-06-01)
The activity of glycerol kinase is rate-limiting in the metabolism of glycerol by cells of Escherichia coli. A mutant strain producing a glycerol kinase resistant to inhibition by fructose-1,6-diphosphate grows faster than its wild-type parent on glycerol as the sole
Ioanna Gouni-Berthold et al.
The British journal of nutrition, 107(11), 1694-1706 (2011-10-15)
Animal and human studies suggest that a malleable protein matrix (MPM) from whey decreases plasma lipid concentrations and may positively influence other components of the metabolic syndrome such as glucose metabolism and blood pressure (BP). The primary objective of this
Daria V Cherkashina et al.
Rejuvenation research, 14(6), 661-667 (2011-10-20)
Abstract Effects of stem and progenitor cells or their compounds on recipient cells are investigated intensively today. In spite of this, their ability to interact with native cells and the final targets affected by them, particularly biochemical parameters that characterize
Laurence Portois et al.
International journal of molecular medicine, 29(6), 1121-1126 (2012-03-20)
Second generation n3-PUFA-depleted rats represent a good animal model of metabolic syndrome as they display several features of the disease such as liver steatosis, visceral obesity and insulin resistance. The goal of our study was to investigate the influence of
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