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F4762

Sigma-Aldrich

Anti-Pig IgG (whole molecule)−FITC antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym(s):

Anti-Porcine IgG (whole molecule)−FITC antibody produced in rabbit, Anti-Swine IgG (whole molecule)−FITC antibody produced in rabbit

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About This Item

MDL number:
MFCD00162751
UNSPSC Code:
12352203
NACRES:
NA.46

biological source

rabbit

Quality Level

200

conjugate

FITC conjugate

antibody form

IgG fraction of antiserum

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

storage condition

protect from light

technique(s)

direct immunofluorescence: 1:32

storage temp.

−20°C

target post-translational modification

unmodified

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General description

Immunoglobulin G (IgG) belongs to immunoglobulin family and consists of gamma (γ) heavy chain in the constant (C) region. IgG is a widely expressed serum antibody. Monomeric structure of IgG constitutes two identical heavy chains and two identical light chains with a molecular weight of 50kDa and 25kDa respectively, the primary structure of this antibody also contains disulfide bonds involved in linking the two heavy chains, linking the heavy and light chains and resides inside the chains. IgG is further subdivided into four classes namely, IgG1, IgG2, IgG3, and IgG4 with different heavy chains, named γ1, γ2, γ3, and γ4, respectively. IgG is produced and released from CD5+B cells, the predominant lymphocytes in the neonatal cell repertoire.

Application

Anti-Pig IgG (whole molecule)−FITC antibody produced in rabbit has been used in direct immunofluorescent labeling.

Biochem/physiol Actions

Immunoglobulin G (IgG) participates in hypersensitivity type II and type III reactions. Maternal IgG is the only antibody transported across the placenta to the fetus. Maternal IgG passively immunizes the infants.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Production of transgenic pigs over-expressing the antiviral gene Mx1
Yan Q, et al.
Cell regeneration (London, England), 3(1), 11-11 (2014)
Structure and function of natural antibodies
Casali P and Schettino EW
Current Topics in Microbiological Immunology, 37(1), 167-179 (1996)
Quanmei Yan et al.
Cell regeneration (London, England), 3(1), 11-11 (2014-11-20)
The myxovirus resistance gene (Mx1) has a broad spectrum of antiviral activities. It is therefore an interesting candidate gene to improve disease resistance in farm animals. In this study, we report the use of somatic cell nuclear transfer (SCNT) to
Jacqueline Fischer-Lougheed et al.
Immunology, 123(3), 390-397 (2007-10-06)
Synthetic anti-idiotypic antibodies represent a potentially valuable tool for the isolation and characterization of B cells that produce xenoantibodies. An anti-idiotypic antibody that binds to a subset of B cells producing antibodies encoded by the variable-region heavy chain 3 (V(H)3)
Human placental Fc receptors and the transmission of antibodies from mother to fetus.
Simister NE and Story CM
Journal of Reproductive Immunology, 37(1), 1-23 (1997)
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