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D4416

Sigma-Aldrich

Deoxyribonucleic acid, High molecular weight from BALB/c mouse, adult

solution

Synonym(s):

Deoxyribonucleic acid, high molecular weight

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About This Item

CAS Number:
MDL number:
UNSPSC Code:
41106310
NACRES:
NA.52

grade

for molecular biology

form

solution

mol wt

>50 kbp

shipped in

wet ice

storage temp.

2-8°C

General description

Highly purified genomic DNA isolated from adult BALB/c mice.

Application

Deoxyribonucleic acid, High molecular weight from BALB/c mouse, adult, is used as a target for amplification by polymerase chain reaction (PCR), restriction endonuclease digestion and as a carrier DNA for precipitations. It may also be used in library construction in bacteriophage lambda vectors and in Southern hybridization. It was used as a standard for DNA quantification by spectrophotometry and as a positive control in TaqMan PCR reactions.
Applications include use as a target for amplification by polymerase chain reaction (PCR), restriction endonuclease digestion and as a carrier DNA for precipitations. May also be used in library construction in bacteriophage lambda vectors and in Southern hybridization.

Components

The DNA is in a solution of 10mM Tris-HCl (pH 7.5) with 0.1mM EDTA.

Other Notes

Highly purified genomic DNA.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Stéphane Hué et al.
Retrovirology, 7(1), 111-111 (2010-12-22)
Xenotropic murine leukaemia viruses (MLV-X) are endogenous gammaretroviruses that infect cells from many species, including humans. Xenotropic murine leukaemia virus-related virus (XMRV) is a retrovirus that has been the subject of intense debate since its detection in samples from humans
Per Levéen et al.
Blood, 106(13), 4234-4240 (2005-09-01)
We have taken advantage of the Cre/lox system to generate a mouse model with inducible deficiency of transforming growth factor beta receptor II (TbetaRII). Using this approach, transforming growth factor beta (TGF-beta) signaling deficiency can be restricted to the hematopoietic
Eleanor R Gray et al.
PloS one, 6(3), e18096-e18096 (2011-03-31)
Several studies have implicated a recently discovered gammaretrovirus, XMRV (Xenotropic murine leukaemia virus-related virus), in chronic fatigue syndrome and prostate cancer, though whether as causative agent or opportunistic infection is unclear. It has also been suggested that the virus can

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