A5043
Adenosine Deaminase from bovine spleen
Type X, buffered aqueous glycerol solution, ≥130 units/mg protein
Synonym(s):
ADA, adenosine aminohydrolase, Adenosine aminohydrolase
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About This Item
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biological source
bovine spleen
Quality Level
type
Type X
form
buffered aqueous glycerol solution
specific activity
≥130 units/mg protein
mol wt
32.5-33 kDa
cation traces
NH4+: ≤10 μg/mg protein
UniProt accession no.
foreign activity
alkaline phosphatase ≤0.01%
guanase ≤0.01%
nucleoside phosphorylase ≤0.1%
storage temp.
2-8°C
Gene Information
cow ... ADA(280712)
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General description
Molecular weight: 32.5-33 kDa
pI: 4.85
pI: 4.85
Application
Adenosine Deaminase from bovine spleen has been used in the immobilization on biostrip for stability and catalytic studies.
Adenosine deaminase is useful in various molecular biology assays, such as glycerol release assays . Adenosine deaminase is a potential target for treatments of combined immunodeficiency disease.
Biochem/physiol Actions
Adenosine deaminase is a purine catabolic enzyme which catalyzes the deamination of adenosine and 2′-deoxyadenosine with approximately equal specificity. It is ubiquitous in mammalian tissue, and deficiency in adenosine deaminase has been associated with severe combined immunodeficiency disease.
Unit Definition
One unit will deaminate 1.0 μmole of adenosine to inosine per min at pH 7.5 at 25 °C.
Physical form
Solution in 50% glycerol, 5 mM potassium phosphate, pH 6.0
Analysis Note
Protein determined by biuret.
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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