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94890

Sigma-Aldrich

Victoria Blue B

for microscopy (Bact., Hist.)

Synonym(s):

Basic Blue 26, Basic Blue B

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About This Item

Empirical Formula (Hill Notation):
C33H32ClN3
CAS Number:
2580-56-5
Molecular Weight:
506.08
Colour Index Number:
44045
Beilstein:
4094258
EC Number:
219-943-6
MDL number:
MFCD00011878
UNSPSC Code:
12352200
PubChem Substance ID:
57653647
NACRES:
NA.25

grade

for microscopy (Bact., Hist.)

Quality Level

100

form

powder

mp

206 °C (dec.) (lit.)

SMILES string

[Cl-].CN(C)c1ccc(cc1)/C(=C2/C=C\C(C=C2)=[N+](\C)C)c3ccc(Nc4ccccc4)c5ccccc35

InChI

1S/C33H31N3.ClH/c1-35(2)27-18-14-24(15-19-27)33(25-16-20-28(21-17-25)36(3)4)31-22-23-32(30-13-9-8-12-29(30)31)34-26-10-6-5-7-11-26;/h5-23H,1-4H3;1H

InChI key

LLWJPGAKXJBKKA-UHFFFAOYSA-N

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Application

Victoria Blue B is used as a nuclear stain in cytology/histochemical applications. Victoria Blue B may be used in the quantitative determination of phospholipids.

Signal Word

Danger

Hazard Statements

H302 - H314 - H317 - H410

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Corr. 1 - Skin Sens. 1

Storage Class Code

8B - Non-combustible, corrosive hazardous materials

WGK

WGK 3

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Some variability factors in the cytomorphological analysis of frozen bull semen.
Galli A, Paloschi L, Pizzi F.
Andrologie, 21, 120-126 (1989)
Dye standards, Part II. 4: Victoria blue B (CI 44045). European Committee for Clinical Laboratory Standards (ECCLS), Subcommittee on Reference Materials for Tissue Stains (SRMTS).
The Histochemical journal, 24(4), 230-232 (1992-04-01)
Seok Joo Doh et al.
Journal of hazardous materials, 154(1-3), 118-127 (2007-11-17)
We have developed photocatalytic TiO2 nanofibers for the treatment of organic pollutants by using electrospinning method. We found that the optimized electrospinning conditions (electric field and flow rate) were 0.9 kV cm(-1) and 50 microL min(-1). After annealing at 550
S Liu et al.
Analytical chemistry, 73(16), 3907-3914 (2001-09-06)
In a near-neutral medium, a combination of heparin with some basic diphenyl naphthylmethane dyes such as victoria blue 4R (VB4R), victoria blue B (VBB), or night blue (NB) can result in a significant enhancement of resonance. Rayleigh scattering (RRS) and
E Schulte et al.
Histochemistry, 88(3-6), 427-433 (1988-01-01)
The aim of this study was to investigate the staining characteristics of Victoria Blue B in alcohol solutions. Cytological specimens (liver and spleen tissue imprints, blood smears) were stained with methanol solutions of commercially available Victoria Blue B-Cl and with
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