PPB022
Tris buffer
Pre-set, pH 7.0, pHast Pack™, powder
Synonym(s):
0.1M Tris
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About This Item
UNSPSC Code:
12352200
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General description
Tris buffer, pH 7.0 is a general buffer that is isotonic and non-toxic in nature due to the presence of tris base. It is suitable for most biochemical and molecular biology applications. Tris has a buffering capacity range of pH 7.0 - 9.0, with a pKa of 8.1 at 25°C.
Application
Tris buffer is used in:
- Western blot
- Protein gel electrophoresis
- cell culture (sterile filter buffer)
- As a buffer component
Features and Benefits
- Fast, ready-to-use buffer
- No need to measure and adjust the pH
- Saves time and minimizes effort in the lab
- Biological tests: free of DNase, RNase, Protease, and Nickase
- Chemical tests: Iron ≤10 ppm, lead ≤5 ppm
Packaging
Foil pouches
Reconstitution
Contents of one pouch, when dissolved in 500 mL of ultrapure water, will yield a 0.1M Tris solution, pH 7.0 at 25 °C. Contents tested to be DNAse, RNAse, Protease, and Nickase free. This product is suitable for use in cell culture and electrophoresis applications.
Storage and Stability
Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.
Other Notes
For additional information on our range of Biochemicals, please complete this form.
Legal Information
pHast Pack is a trademark of Sigma-Aldrich Co. LLC
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
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