93321

TRIS Glycine buffer solution

BioUltra, 10× concentrate

• Synonym(s): Blotting buffer solution
• UNSPSC Code: 41105319

Properties

• product line: BioUltra
• Quality Level: 100
• form: liquid
• quality: 10× concentrate
• composition: glycine, 1.92 M ; TRIS, 0.25 M
• impurities: insoluble matter, passes filter test; proteases, none detected
• ign. residue: ≤0.2%
• pH: 8.5-8.7
• anion traces: sulfate (SO₄^2-): ≤50 mg/kg
• cation traces: Al: ≤5 mg/kg; As: ≤0.1 mg/kg; Ba: ≤5 mg/kg; Bi: ≤5 mg/kg; Ca: ≤10 mg/kg; Cd: ≤5 mg/kg; Co: ≤5 mg/kg; Cr: ≤5 mg/kg; Cu: ≤5 mg/kg; Fe: ≤5 mg/kg; K: ≤50 mg/kg; Li: ≤5 mg/kg; Mg: ≤5 mg/kg; Mn: ≤5 mg/kg; Mo: ≤5 mg/kg; NH₄⁺: ≤200 mg/kg; Na: ≤500 mg/kg; Ni: ≤5 mg/kg; Pb: ≤5 mg/kg; Sr: ≤5 mg/kg; Zn: ≤5 mg/kg
• λ: neat
• UV absorption: λ: 260 nm A_max: 0.1; λ: 280 nm A_max: 0.1

Description

Application

Trizma is used in the formulation of buffer solutions in the pH range between 7.5 and 8.5. Tris buffer solutions are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification. TRIS Glycine buffer solution, 10X concentrate is diluted to a working concentration of 1X and pH adjusted as required. TRIS Glycine buffer (TGB) is frequently used in gel electrophoresis and ion-exchange chromatography applications.

Other Notes

Electrophoretic transfer of protein from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves