52062

LB-Agar, high salt

suitable for microbiology, NutriSelect^® Plus

• Synonym(s): Luria Bertani Agar, high salt
• UNSPSC Code: 41171605
• NACRES: NA.85

Properties

• sterility: non-sterile
• Quality Level: 200
• form: powder
• shelf life: limited shelf life, expiry date on the label
• composition: agar, 15 g/L ; casein enzymic hydrolysate, 10 g/L ; sodium chloride, 10 g/L ; yeast extract, 5 g/L
• manufacturer/tradename: NutriSelect^® Plus
• technique(s): microbiological culture: suitable
• final pH: 7.5±0.2 (25 °C)
• application(s): clinical testing; food and beverages; life science and biopharma; microbiology
• suitability: nonselective for Escherichia coli; nonselective for bacteria (General Media); nonselective for coliforms

Description

General description

LB-Agar, high salt is a growth media used in microbiology. It supports Escherichia coli growth to an optical density at 600 nm (OD₆₀₀) of 7.

Application

LB-Agar, high salt may be used as a growth media in the determination of phylogenetic groups (A, B1, B2, and D) of Escherichia coli bacteria using triplex PCR technique.

Luria Media are used for the cultivation and maintenance of recombinant strains of E. coli for genetic and molecular studies.

Preparation Note

Suspend 40 g in 1000 ml of purified water. Heat with frequent agitation and boil for one minute. Sterilize at 121°C for 15 minutes. Dispense as desired.

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves