410217

2′,7′-Dichlorofluorescein

ACS reagent

• Synonym(s): Dichlorofluorescein; Fluorescein 27
• Empirical Formula (Hill Notation): C₂₀H₁₀Cl₂O₅
• CAS Number: 76-54-0
• Molecular Weight: 401.20
• Beilstein: 58009
• EC Number: 200-968-6
• MDL number: MFCD00005047
• UNSPSC Code: 12171500
• PubChem Substance ID: 24865700
• NACRES: NA.47

Properties

• grade: ACS reagent
• Quality Level: 200
• form: powder
• technique(s): titration: suitable
• color: orange to red-brown, powder
• mp: 280 °C (dec.) (lit.)
• solubility: alcohol: passes test
• density: 0.790 g/cm³
• λmax: 509 nm
• suitability: passes test for adsorption indicator
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: Oc1cc2Oc3cc(O)c(Cl)cc3C4(OC(=O)c5ccccc45)c2cc1Cl
• InChI: 1S/C20H10Cl2O5/c21-13-5-11-17(7-15(13)23)26-18-8-16(24)14(22)6-12(18)20(11)10-4-2-1-3-9(10)19(25)27-20/h1-8,23-24H
• InChI key: VFNKZQNIXUFLBC-UHFFFAOYSA-N

Description

General description

2′,7′-Dichlorofluorescein is an oxidation-sensitive fluorescent probe.

Application

2′,7′-Dichlorofluorescein has been used to measure ROS (reactive oxygen species) formation in cells. It has been used as a fluorogenic substrate to study the peroxidase activity of saliva samples and extracts obtained from submandibular glands.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves