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Key Documents

ST1549

Sigma-Aldrich

Anti-FASN Mouse mAb (3F2-1F3)

liquid, clone 3F2-1F3, Calbiochem®

Synonym(s):

Anti-Fatty Acid Synthase

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About This Item

UNSPSC Code:
12352203

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

3F2-1F3, monoclonal

form

liquid

does not contain

preservative

species reactivity

human

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

isotype

IgG1

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... FASN(2194)

General description

Anti-FASN, mouse monoclonal, clone 3F2-1F3, recognizes FASN protein in 293 and HeLa cells and in human breast carcinoma tissue. It is validated for ELISA, WB, ICC, and IHC (paraffin sections).
Purified mouse monoclonal antibody. Recognizes the FASN protein.
Recognizes the FASN protein in 293 and HeLa cells and human breast carcinoma tissue.

Immunogen

Full-length, recombinant human FASN (aa 1-440) expressed as GST fusion protein

Application




ELISA (see coments)
Immunoblotting (1-5 g/ml)
Immunocytochemistry (10 g/ml)
Paraffin Sections (10 g/ml)

Warning

Toxicity: Regulatory Review (Z)

Physical form

In PBS, pH 7.2.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Positive Control
293 cells, HeLa cells, Human breast carcinoma tissue

Other Notes

For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Kristiansen, G., et al. 2010. Br. J. Cancer102, 1736.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Boubacar Benziane et al.
Journal of lipid research, 58(12), 2324-2333 (2017-10-27)
Diacylglycerol kinases (DGKs) regulate the balance between diacylglycerol (DAG) and phosphatidic acid. DGKζ is highly abundant in skeletal muscle and induces fiber hypertrophy. We hypothesized that DGKζ influences functional and metabolic adaptations in skeletal muscle and whole-body fuel utilization. DAG

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