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MABN110

Sigma-Aldrich

Anti-pro-BDNF Antibody, clone 5D4.1

clone 5D4.1, 1 mg/mL, from mouse

Synonym(s):

Brain-derived neurotrophic factor, Abrineurin

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

5D4.1, monoclonal

species reactivity

mouse, human, rat

concentration

1 mg/mL

technique(s)

immunohistochemistry: suitable (paraffin)
western blot: suitable

isotype

IgG1κ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... BDNF(627)

General description

Brain Derived Neurotrophic Factor (BDNF) is a secreted protein that promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. It is a major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. It is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. Expression of this gene is reduced in both Alzheimer’s and Huntington disease patients. BDNF may play a role in the regulation of stress response and in the biology of mood disorders. BDNF exists as monomers or homodimers and binds to TRKB/NTRK2 receptors.

Specificity

The antibody reacts to the pro form of BDNF

Immunogen

GST-tagged recombinant protein corresponding to Human pro-BDNF.

Application

Research Category
Neuroscience
Research Sub Category
Developmental Neuroscience
This Anti-pro-BDNF Antibody, clone 5D4.1 is validated for use in Western Blotting and Immunohistochemistry (Paraffin) for the detection of pro-BDNF.
Western Blotting Analysis: A 0.5 µg/mL dilution from a representative lot detected 10 µg of recombinant protein Pro-BDNF but not recombinant protein BDNF.
Immunohistochemistry Analysis: A 1:2000 dilution from a representative lot detected Pro-BDNF in human cerebral cortex and thalamic tissue.

Quality

Evaluated by Western Blotting in Rat Brain cell lysate.

Western Blotting Analysis: A 0.5 µg/mL diultion of this antibody detected pro-BDNF in 200 µg of Rat Brain cell lysate.

Target description

~ 34 kDa observed (Neurochemical Journal, 2012, Vol. 6, No. 4, pp 260-264).

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Federico José Barreda Tomás et al.
International journal of molecular sciences, 21(5) (2020-02-29)
Brain-derived neurotrophic factor (BDNF) is a major neuronal growth factor that is widely expressed in the central nervous system. It is synthesized as a glycosylated precursor protein, (pro)BDNF and post-translationally converted to the mature form, (m)BDNF. BDNF is known to

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