MAB390
Anti-p75 LNGFR Antibody, Saporin conjugated, clone 192
clone 192, Chemicon®, from mouse
Synonym(s):
CD271 antigen, Low affinity neurotrophin receptor p75NTR, Low-affinity nerve growth factor receptor, NGF receptor, low affinity nerve growth factor receptor, nerve growth factor receptor, nerve growth factor receptor (TNFR superfamily, member 16), p75 IC
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
conjugate
saporin
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
192, monoclonal
species reactivity
rat
should not react with
human, mouse
packaging
antibody small pack of 25 μg
manufacturer/tradename
Chemicon®
technique(s)
immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... NGFR(4804)
General description
The Low Affinity Nerve Growth Factor Receptor (also called the LNGFR or p75 neurotrophin receptor) is one of the two receptor types for the neurotrophins, a family of protein growth factors which stimulate neuronal cells to survive and differentiate. The neurotrophins are comprised of four proteins, all of which bind to the LNGFR: Nerve Growth Factor (NGF), Brain Derived Neurotrophic Factor (BDNF), Neurotrophin-3 (NT-3), and Neurotrophin-4 (NT-4. Nerve growth factor, the prototypical growth factor, is a protein secreted by a neuron′s target. NGF is critical for the survival and maintenance of sympathetic and sensory neurons. NGF is released from the target cells, binds to and activates its high affinity receptor (TrkA), and is internalized into the responsive neuron. The NGF/TrkA complex is subsequently trafficked back to the cell body. This movement of NGF from axon tip to soma is thought to be involved in the long-distance signaling of neurons.
Specificity
Recognizes the rat NGF receptor (p75, low affinity Neurotrophin Receptor). MAB365 is the monoclonal antibody used for MAB390-SAP.
Immunogen
p75-expressing cells
Application
Immunohistochemistry (Paraffin):
A previous lot of this antibody was used in IH(P).
Immunocytochemistry:
A previous lot of this antibody was used in IC.
Optimal working dilutions must be determined by end user.
A previous lot of this antibody was used in IH(P).
Immunocytochemistry:
A previous lot of this antibody was used in IC.
Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurodegenerative Diseases
Neurochemistry & Neurotrophins
Neurodegenerative Diseases
Neurochemistry & Neurotrophins
This Anti-p75 Low Affinity Nerve Growth Factor Receptor Antibody, Saporin conjugated, clone 192 is validated for use in ICC, IHC(P), FUNC for the detection of p75 Low Affinity Nerve Growth Factor Receptor.
Physical form
Protein A purified
Saporin Conjugated. 192-IgG-SAP is sterile filtered and presented in Dulbecco′s PBS with no preservative.
Storage and Stability
Centrifuge material at low speed in microfuge to ensure all of the solution is at the bottom of the tube. Vortex gently. Long term storage at -70ºC or below. Short term storage at -20ºC or 2-8ºC with appropriate antibacterial agent if compatible with the intended application. The sterile conjugate is stable at 2-8ºC for several months. Avoid repeated freezing and thawing. Glycerol (1:1) can be added for additional stability if compatible with the intended application. This immunotoxin may be handled safely using standard laboratory procedures as the cross-species reactivity between these anti-rat p75 antibodies and the human form of p75 is negligible.
Analysis Note
Control
Brain tissue
Brain tissue
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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T Deacon et al.
Cell transplantation, 8(1), 111-129 (1999-05-25)
The anatomical specificity of axon growth from fetal pig septal xenografts was studied by transplanting septal cells from E30-35 pig fetuses into cholinergic deafferented (192-IgG-saporin-infused) rats or into aged rats (> 18 months). Cell suspensions (100,000 cells/microl) were injected bilaterally
R Lemke et al.
Neuroscience letters, 267(1), 53-56 (1999-07-10)
In adult intact rat brain, leukemia inhibitory factor (LIF) mRNA has been found to be constitutively expressed in basal forebrain cholinergic neurons. To reveal a functional role of LIF in neurodegenerative events, the cellular expression pattern of LIF was determined
P E Potter et al.
Neuropharmacology, 38(4), 579-586 (1999-04-30)
Cholinergic neurons projecting from the medial septum to the hippocampus were lesioned with the selective neurotoxin 192 IgG-saporin. Injection of 300 ng of 192 IgG-saporin into the medial septum produced a 60% decrease in choline acetyltransferase activity. M1 muscarinic receptor
Christiana M Cooper-Kuhn et al.
Journal of neuroscience research, 77(2), 155-165 (2004-06-24)
Adult neurogenesis has been shown to be regulated by a multitude of extracellular cues, including hormones, growth factors, and neurotransmitters. The cholinergic system of the basal forebrain is one of the key transmitter systems for learning and memory. Because adult
Selective immunolesions of hippocampal cholinergic input fail to impair spatial working memory.
McMahan, R W, et al.
Hippocampus, 7, 130-136 (1997)
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