MAB350
Anti-Enkephalin Antibody, clone NOC1
culture supernatant, clone NOC1, Chemicon®
Synonym(s):
Anti-CBF, Anti-CBF2, Anti-HSP-CBF, Anti-NOC1
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
mouse
Quality Level
antibody form
culture supernatant
antibody product type
primary antibodies
clone
NOC1, monoclonal
species reactivity
guinea pig, rabbit, rat, hamster
manufacturer/tradename
Chemicon®
technique(s)
immunocytochemistry: suitable
immunohistochemistry: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... CEBPZ(10153)
Specificity
Met5- and Leu5-enkephalin. Displays about 40% cross-reactivity with C-terminal extended Met-enkephalin hexapeptides and 7% cross-reactivity with the extended heptapeptide (-Arg-Phe-OH) but does not recognize pro-enkephalin or other endogenous peptides (Cuello, 1984). By immunohistochemistry the antibody lables all sites that are known to exppress enkephalin and shows no cross-reactivity to beta-endorphin or dynorphin (Cuello, 1984; MacMillan and Cuello, 1986).
Immunogen
Leu5-enkephalin conjugated to BSA.
Application
Detect Enkephalin using this Anti-Enkephalin Antibody, clone NOC1 validated for use in IC, IH.
Immunohistochemistry: 1:100-1:400 on 10 μm cryostat sections of rat and guinea-pig brain stem using fluorescently labled secondary antibodies for detection (Cuello, 1984; MacMillan and Cuello, 1986).
Immunocytochemistry: 1:300 on primary cultures of rat striatal and hippocampal neurons using a Cy2 or Cy3 conjugated secondary antibody for detection (Saudou, 1998).
Radioimmunoassay (Cuello, 1984)
Optimal working dilutions must be determined by the end user.
Immunocytochemistry: 1:300 on primary cultures of rat striatal and hippocampal neurons using a Cy2 or Cy3 conjugated secondary antibody for detection (Saudou, 1998).
Radioimmunoassay (Cuello, 1984)
Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neuroinflammation & Pain
Hormones & Receptors
Neuroinflammation & Pain
Hormones & Receptors
Physical form
Lyophilized Supernatant, preservative free.
Storage and Stability
Lyophilized antibody can be kept at 2-8°C for up to 3 months and should be kept at -20°C for long term storage. Reconstitute with 100μL sterile DI water. Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2
Storage Class Code
11 - Combustible Solids
WGK
WGK 2
Certificates of Analysis (COA)
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F Saudou et al.
Cell, 95(1), 55-66 (1998-10-20)
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