251505-M

DAF-2 DA

A cell-permeable derivative of DAF-2 that is hydrolyzed to DAF-2 by intracellular esterases.

• Synonym(s): DAF-2 DA, 4,5-Diaminofluorescein Diacetate
• Empirical Formula (Hill Notation): C₂₄H₁₈N₂O₇
• CAS Number: 205391-02-2
• Molecular Weight: 446.41
• MDL number: MFCD05865101
• UNSPSC Code: 12352200

Properties

• Quality Level: 100
• Assay: ≥98% (HPLC)
• form: solution
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; protect from light
• color: pale yellow
• fluorescence: λ_ex ~495 nm; λ_em ~515 nm
• storage temp.: −20°C
• InChI: 1S/C24H18N2O7/c1-11(27)30-13-3-5-16-21(7-13)32-22-8-14(31-12(2)28)4-6-17(22)24(16)18-10-20(26)19(25)9-15(18)23(29)33-24/h3-10H,25-26H2,1-2H3
• InChI key: PTSUYDXEEKDBQU-UHFFFAOYSA-N

Description

General description

A cell-permeable derivative of DAF-2 (Cat. No. 251500) that is hydrolyzed to DAF-2 by intracellular esterases. Can be used in fluorescence microscopy to measure real-time changes in NO levels in vivo.

A cell-permeable derivative of DAF-2 (Cat. No. 251500) that is hydrolyzed to DAF-2 by intracellular esterases (excitation maximum: 495 nm; emission maximum: 515 nm). Can also be used in fluorescence microscopy.

Biochem/physiol Actions

Primary Target
Used to measure real-time changes in NO levels

Warning

Toxicity: Irritant (B)

Physical form

A 5 mM (1 mg/450 µl) solution in DMSO.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Other Notes

Berkels, R., et al. 2000. Cell Calcium27, 281.
Kojima, H., et al. 1998. Anal. Chem. 70, 2446.
Kojima, H., et al. 1998. Chem. Pharm. Bull. 46, 373.
Nakatsubo, N., et al. 1998. FEBS Lett. 427, 263.

Legal Information

Manufactured by Daiichi Pure Chemicals Co., Ltd

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): 188.6 °F - (Dimethylsulfoxide)
• Flash Point(C): 87 °C - (Dimethylsulfoxide)