176872

Angiotensin Converting Enzyme-2, His•Tag^®, Human, Recombinant, NSO Cells

• Synonym(s): ACE Homolog, ACEH, ACE-2
• UNSPSC Code: 12352202

Properties

• recombinant: expressed in NSO cells
• Quality Level: 100
• Assay: ≥90% (SDS-PAGE)
• form: liquid
• specific activity: ≥0.8 units/μg protein
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; avoid repeated freeze/thaw cycles
• impurities: ≤1.0 EU/μg Endotoxins (EU/μg ACE-2)
• shipped in: wet ice
• storage temp.: −70°C

Description

General description

Recombinant, human ACE-2 ectodomain (amino acids 1-740) fused at the C-terminus to a His•Tag sequence and expressed in NSO cells. ACE-2 cleaves angiotensins I and II as a carboxypeptidase and plays an important role in regulating cardiovascular and renal functions. Native ACE-2 is an integral membrane zinc metalloprotease whose activity is inhibited by EDTA. By SDS-PAGE, the apparent molecular mass of the glycosylated protein is ~120 kDa under either reducing or non-reducing conditions.

Recombinant, human ACE-2 ectodomain (amino acids 1-740) fused at the C-terminus to a His•Tag sequence and expressed in NSO cells. Native ACE-2 is an integral membrane zinc metalloprotease that can be inhibited by EDTA. ACE-2 cleaves angiotensins I and II. Plays an important role in regulating cardiovascular and renal functions. By SDS-PAGE, the apparent molecular mass of the glycosylated protein is ~120 kDa under either reducing or non-reducing condition.

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of enzyme that will cleave 1 nmol MCA-YVADAPK(DNP)-OH per min at 25°C, pH 7.5.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Other Notes

Tikellis, C., et al. 2003. Hypertension41, 390.
Tipnis, S.R., et al. 2000. J. Biol. Chem.275, 33238.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable