1.52258
Chromolith® WP 300 Protein A (monolithic) HPLC Columns
L × I.D. 25 mm x 4.6 mm HPLC Column
Synonym(s):
Chromolith® WP 300 Protein A 25-4.6 HPLC column
About This Item
Recommended Products
product name
Chromolith® WP 300 Protein A, L × I.D. 25 mm x 4.6 mm HPLC column
material
PEEK column
product line
Chromolith®
feature
endcapped: no
endcapped: no
packaging
1 ea of
parameter
2 - 45 °C temp. range
2-45 °C temp. range
200 bar pressure
200 bar pressure
technique(s)
HPLC: suitable
HPLC: suitable
LC/MS: suitable
column L × I.D.
2.5 cm × 4.6 mm
surface area
120 m2/g
matrix
monolithic silica
monolithic silica
matrix active group
Protein A bonding phase
Protein A bonding phase
pore size
2 μm macroporosity
2 μm macroporosity
30 nm mesoporosity
30 nm mesoporosity
operating pH range
1.5-7.5
operating pH
1.5-7.5
separation technique
affinity
storage temp.
2-8°C
2-8°C
Looking for similar products? Visit Product Comparison Guide
General description
Chromolith® WP 300 Protein A 25-4.6 columns are made of monolithic silica with a characteristic bimodal pore structure.
Key Benefits:
—Rapid and accurate titer determination across a wide range of injection volumes
—Consistent binding efficiencies even at high flow rates
—Expanded column lifetimes
Learn more:
Brochure: Race through your Separations on Any System. Chromolith® Monolithic Silica HPLC Columns
Chromolith® WP 300 Protein A is available in 2 mm I.D. providing improved efficiency, sensitivity, and enhanced MS-compatibility.
Flyer: Rapid and Robust Antibody Quantitation - Chromolith® WP 300 Protein A HPLC columns
All Supelco HPLC Columns, including monolithic Chromolith® columns, are fully compatible to all HPLC and UHPLC Instruments.
Supelco HPLC Products: The Best Choice for Any LC Instrument
Legal Information
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Articles
Silica monoliths with larger mesopores are a powerful tool for the fast separation of biomolecules including mAbs. The immobilization of protein A on epoxy-modified widepore (WP) silica rods enabled highly reproducible and fast, one minute, separations of mAbs. The Chromolith® WP 300 is also available with a protein A phase.
Related Content
HPLC separates and identifies large biomolecules like proteins and peptides by pumping the sample through a sorbent-packed column.
Analytical Chromolith® HPLC columns for Bio applications, 300A mesopores: RP-18, RP-8, RP-4, Prot-A, Epoxy
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service