1.15938

Hematoxylin monohydrate (C.I.75290)

for microscopy Certistain^®

• Synonym(s): Hematoxylin monohydrate (C.I.75290)
• Empirical Formula (Hill Notation): C₁₆H₁₄O₆ · xH₂O
• Molecular Weight: 302.28 (anhydrous basis)
• UNSPSC Code: 41116130
• EC Index Number: 208-237-3
• NACRES: NA.21

Properties

• Quality Level: 400
• form: solid
• composition: Dye, ≥95% spectrophotometric assay
• IVD: for in vitro diagnostic use
• mp: 140 °C (Elimination of water of crystallisation)
• solubility: slightly soluble at 20 °C
• bulk density: 500 kg/m³
• εmax: 155-165 at 290-292 nm in acetonitrile at 0.05 g/L
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: 2-30°C

Description

General description

Hämatoxylin-Monohydrat (C.I. 75290) for microscopy Certistain^®, is a dye for the specific staining of DNA in the nuclei. It is used in cytology and histology. Nuclei are stained dark blue, dark violet to black. Hematoxylins are mixed with trivalent positively charged metal salts and build up the so-called hematoxylin lakes, used for the selective staining of nuclei (DNA). Certistain^® is the quality group for seleceted high quality dyes that fulfill all quality requirements for IVD and CE registration. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Analysis Note

Dye content (spectrophotometrically): ≥ 95 %
Identity (UV/VIS-Spectrum): passes test
Absorption maximum λ max (acetonitrile): 290 - 292 nm
Spec. Absorptivity A 1%/1cm (λ_max; 0.05 g/l; acetonitrile): 155 - 165
Haematein (spectrophotometrically): ≤ 0.1 %
Water (according to Karl Fischer): 5 - 6 %
Suitability for microscopy: passes test

Legal Information

CERTISTAIN is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H319
• Precautionary Statements: P264 - P280 - P305 + P351 + P338 - P337 + P313
• Hazard Classifications: Eye Irrit. 2
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1