1.05642
HPTLC plates, Silica gel 60 F₂₅₄
pkg of 50 plates, plate L × W 20 cm × 10 cm, glass support
Synonym(s):
HPTLC Silica Gel Plates
About This Item
Recommended Products
material
glass support
silica gel 60 matrix
Quality Level
feature
binder Organic Polymer
fluorescent indicator
packaging
pkg of 50 plates
technique(s)
thin layer chromatography (TLC): suitable
layer thickness
200 μm
plate L × W
20 cm × 10 cm
particle size
5-6 μm
pore size
60 Å medium pore diameter
storage temp.
2-30°C
Related Categories
General description
Application
Linkage
Analysis Note
Pore volume (N₂-isotherm): 0.74 - 0.84 ml/g
d 50 (laser diffraction, size distribution): 5 - 7 µm
Layer thickness: 150 - 200 µm
Deviation of layer thickness per plate: ≤ 35 µm
Chromatographic testings:
A) Colour test
hRf-values
- bleu vif organol, colour test, lipophile:
17 - 22
- Ceres Black G, colour test, lipophile: 38 - 43
- ceres Violet brn, colour test, lipophile: 58 - 64
separation number
- colourtest, lipophile:
≥ 10.0
B) Steroid test
hRf-values
- hydrocortisone, steroid test:
23 - 29
- Reichstein S, steroid test: 36 - 45
- methyltestosterone, steroid test: 43 - 52
separation number
- steroid test:
≥ 10.5
Typical value determined on a conditioned plate
Eluent A) Toluene (45% rel.humidity)
Eluent B) Ethyl acetate/Toluene (95+5 v/v; 20% rel.humidity)
Certificates of Analysis (COA)
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Articles
In this article we present several HPTLC applications and analytical standards for ginsenosides.
HPTLC is an efficient method to determine Steviol Glycosides content in Stevia using our HPTLC plates and reference materials, including a new introduced Stevia extract reference material.
Protocols
Powdered black pepper was chosen as example for dietary supplements, being a source for pierine. It is tested according to the current United States Pharmacopeia (USP) guidelines using HPTLC and HPLC.
In the present study we demonstrate compliance of silica gel 60 F254 HPTLC plates with USP requirements, and how HPTLC plates are suitable for the Sildenafil citrate-Limit of Imidazole test.
Effective analysis of passion flower by HPTLC fingerprinting, in conjunction with a comprehensive range of related reference materials, enables fast and efficient identification of this plant material.
Separation of Caffiene
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