07-178
Anti-Hox A9 Antibody
Upstate®, from rabbit
Synonym(s):
Anti-ABD-B, Anti-HOX1, Anti-HOX1.7, Anti-HOX1G
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
species reactivity
mouse, human
manufacturer/tradename
Upstate®
technique(s)
electrophoretic mobility shift assay: suitable
immunocytochemistry: suitable
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... HOXA9(3205)
General description
HOXA9 is a transcription factor with a central role in both haemopoiesis and leukaemia. High levels of HOXA9 expression in haemopoietic cells is a characteristic feature of acute myeloid leukaemia (AML), and may be sufficient to cause this disease. Overexpression of Hoxa-9 markedly expands hematopoietic stem cells. HOXA9 expression changes dramatically with age - a uniformly low level of expression during early adulthood is replaced by a frequently very high expression in adults over sixty.
Specificity
Based on a BLAST search, the antibody will likely cross react with Newt, Coelacanth, Dog, Chicken, and Rat Hox A9; Antelope, Mouse and Guinea Pig Hox 1.7; Frog, human and Zebrafish Hox A-9B; and Kenyan Clawed Frog Hox B.1
Hox A9
Immunogen
GST fusion protein corresponding to residues 194-272 of mouse Hox A9
Application
Detect Hox A9 with Anti-Hox A9 Antibody (Rabbit Polyclonal Antibody), that has been shown to work in EMSA, WB, ICC.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Transcription Factors
Quality
Routinely evaluated by immunoblot on whole cell lysates of Hoxa9 immortalized HF1 myeloid progenitor cells
Target description
36kDa
Physical form
Format: Purified
Protein A chromatography
Protein A purified immunoglobulin presented in 0.02M phosphate buffer, pH 7.6, 0.25M NaCl, and 0.1% Sodium Azide before the addition of glycerol to 30%.
Storage and Stability
2 years at -20°C
Analysis Note
Control
Hoxa9 immortalized HF1 cells
Hoxa9 immortalized HF1 cells
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Find documentation for the products that you have recently purchased in the Document Library.
Isolated Hoxa9 overexpression predisposes to the development of lymphoid but not myeloid leukemia.
Beachy, SH; Onozawa, M; Silverman, D; Chung, YJ; Rivera, MM; Aplan, PD
Experimental Hematology null
K R Calvo et al.
Molecular and cellular biology, 20(9), 3274-3285 (2000-04-11)
The genes encoding Hoxa9 and Meis1 are transcriptionally coactivated in a subset of acute myeloid leukemia (AML) in mice. In marrow reconstitution experiments, coexpression of both genes produces rapid AML, while neither gene alone generates overt leukemia. Although Hoxa9 and
Ryo Miyamoto et al.
eLife, 10 (2021-07-27)
HOXA9 is often highly expressed in leukemias. However, its precise roles in leukemogenesis remain elusive. Here, we show that HOXA9 maintains gene expression for multiple anti-apoptotic pathways to promote leukemogenesis. In MLL fusion-mediated leukemia, MLL fusion directly activates the expression
Leukemic fusion genes MLL/AF4 and AML1/MTG8 support leukemic self-renewal by controlling expression of the telomerase subunit TERT.
A Gessner,M Thomas,P Garrido Castro,L Buchler,A Scholz,T H Brummendorf,N Martinez Soria et al.
Leukemia null
Integrative epigenomic analysis identifies biomarkers and therapeutic targets in adult B-acute lymphoblastic leukemia.
Geng, H; Brennan, S; Milne, TA; Chen, WY; Li, Y; Hurtz, C; Kweon, SM; Zickl, L; Shojaee et al.
Cancer Discovery null
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