Recommended Products
type
for DNA synthesis
Quality Level
product line
Proligo Reagents
form
liquid
storage condition
dry at room temperature
impurities
≤100 ppm water content (Karl Fischer)
color
clear
suitability
conforms to structure (GC, Formulation)
compatibility
configured for (ÄKTA® and OligoPilot®)
storage temp.
room temp
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Application
Cap A solution is used after the phosphoramidite reaction/coupling step. This solution aborts chains bearing unreacted 5′hydroxyl groups due to failure of reaction with activated phosphoramidite (typically, 1 to 2% of growing oligonucleotide chains in each synthesis cycle). These unreacted 5′hydroxyl groups are capped with an acetyl group and thus rendered unreactive for subsequent synthesis steps.
Preparation Note
Acetonitrile / N-Methylimidazole 8/2 (v/v)
Other Notes
Cap A solution, containing acetonitrile (ACN) and N-methylimidazole (NMI), is mixed in situ with Cap B1 and Cap B2 solution during the capping (acetylation) reaction. Pyridine is applied as a mild base while NMI provides a powerful acylation catalyst.
Legal Information
OligoPilot is a registered trademark of Cytiva
ÄKTA is a registered trademark of Cytiva
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 2 - Repr. 2 - Skin Corr. 1B
Storage Class Code
3 - Flammable liquids
WGK
WGK 2
Flash Point(F)
35.6 °F
Flash Point(C)
2 °C
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