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G005561

HPLC Analysis of B-Trichothecenes on Ascentis® Express C18

application for HPLC

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technique(s)

HPLC: suitable

test parameters

column: Ascentis Express C18, 10 cm x 3 mm I.D., 2.7 μm particles (53814-U)
mobile phase: [A] water:acetonitrile:methanol (92:4:4); [B] acetonitrile
gradient: 0% B held for 5 minutes, to 8% B in 3 minutes, 8% B held for 2 minutes
flow rate: 0.8 mL/min
pressure: 4061 psi (280 bar)
column temp.: 35 °C
detector: UV, 220 nm
injection: 40 μL
sample: 2 ppm each in water:methanol:acetonitrile (92:4:4)

suitability

application for HPLC

application(s)

agriculture
food and beverages

Analysis Note

Mycotoxins are toxic secondary metabolites produced by fungi. Those chemicals exist in food as a result of fungal infection of crops. Mycotoxins have strong resistance to decomposition and digestion, so they remain in the food chain in meat and dairy products. They also resist to temperature, such as cooking and freezing. Their effects on human and animal health include death, cancer, weakened immune systems and as allergens or irritants.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

analytical column

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