G005530

HPLC Analysis of Bisphenol A in Water on Ascentis^® Express C18 after SPE using Supelclean^™ ENVI-18

application for HPLC

Properties

• technique(s): HPLC: suitable
• test parameters: SPE tube/cartridge: Supelclean ENVI-18, 500 mg/6 mL glass SPE tube (54331-U); condition: 1 mL 1% formic acid in acetonitrile, then 1 mL water; sample: 5 mL spiked water blank, concentration of 0.2 μg/mL; elution: 2 mL 1% formic acid in acetonitrile; eluate post-treatment: 1 mL aliquot of eluent, and evaporate/reconstitute to 0.5 mL in acetonitrile; column: Ascentis Express C18, 10 cm x 2.1 mm I.D., 2.7 μm particles (53823-U); mobile phase: [A] water; [B] acetonitrile; (60:40, A:B); ; ; flow rate: 0.4 mL/min; pressure: 3270 psi (256 bar); column temp.: 35 °C; detector: UV, 230 nm; injection: 1 μL
• suitability: application for HPLC
• application(s): environmental

Description

Analysis Note

Bisphenol A is a contaminant that can leach into aqueous solutions from polycarbonate plastics or metal cans; it may impact human development and reproduction. This application demonstrates the extraction and analysis of bisphenol A from water.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Supelclean is a trademark of Sigma-Aldrich Co. LLC

Analyte

Bisphenol A, ≥99%