568223-U
Discovery® BIO Wide Pore C18 HPLC Column
5 μm particle size, L × I.D. 25 cm × 4.6 mm
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About This Item
UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52
material
stainless steel column
Quality Level
Agency
suitable for USP L1
product line
Discovery®
feature
endcapped
manufacturer/tradename
Discovery®
packaging
1 ea of
extent of labeling
9.2% Carbon loading
parameter
0-70 °C temperature
400 bar pressure (5801 psi)
technique(s)
HPLC: suitable
LC/MS: suitable
L × I.D.
25 cm × 4.6 mm
surface area
100 m2/g
surface coverage
3.6 μmol/m2
matrix
silica particle platform
fully porous particle
matrix active group
C18 (octadecyl) phase
particle size
5 μm
pore size
300 Å
operating pH
2-8
separation technique
reversed phase
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General description
Peptide maps generated by RP-HPLC provide valuable information about protein structure, stability, and purity. To be effective, the RP-HPLC column must be able to resolve a high percentage of the peptides in the sample. The more peptides, the better the information. Discovery BIO Wide Pore C18 gives unsurpassed RP-HPLC resolution of peptide maps from enzymatic digests. The improvements in silica and bonded-phase chemistry we have incorporated into the Discovery BIO Wide Pore line improve resolution by increasing efficiency and reducing the peak tailing. An added benefit to this is the ability to analyze peptides without TFA in the mobile phase, thereby increasing the LC-MS signal.
Legal Information
Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany
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Hannah F Speer et al.
Journal of animal science, 98(9) (2020-08-28)
This study assessed the relative bioavailability of guanidinoacetic acid (GAA) in cattle. Seven ruminally cannulated Holstein steers (initial body weight of 280 kg) were used in an experiment with a 5 × 5 Latin square design; the two additional steers
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