ZRB2674

Anti-POMC Antibody, clone 2J4 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): Corticotropin-lipotropin, Pro-opiomelanocortin
• UNSPSC Code: 12352203

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 2J4, recombinant monoclonal
• description: recombinant, expressed in HEK 293 cells
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 29.42 kDa; observed mol wt ~35 kDa
• purified by: using Protein A
• species reactivity: rat, human
• species reactivity (predicted by homology): mouse
• packaging: antibody small pack of 25
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• technique(s): affinity binding assay: suitable; immunofluorescence: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgG
• epitope sequence: C-terminal half
• Protein ID accession no.: NP_000930
• UniProt accession no.: P01189
• shipped in: ambient
• storage temp.: 2-8°C
• Gene Information: human ... POMC(5443)

Description

General description

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 2J4 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects Pro-opiomelanocortin (POMC). It targets an epitope within 22 amino acids from the C-terminal half.

Immunogen

KLH-conjugated linear peptide corresponding to 22 amino acids from the C-terminal half of human Pro-opiomelanocortin (POMC).

Application

Quality Control Testing

Evaluated by Western Blotting in Rat pituitary tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected POMC in Rat pituitary tissue lysate.

Tested Applications

Western Blotting Analysis: A 1:5,000 dilution from a representative lot detected POMC in Human pituitary tissue lysate.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected POMC in Rat pituitary gland tissue sections.

Affinity Binding Assay: A representative lot of this antibody bound POMC peptide with a KD of 1.0 x 10-12 in an affinity binding assay.

Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected POMC in Rat pituitary gland tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Pro-opiomelanocortin (UniProt: P01189; also known as POMC, Corticotropin-lipotropin) is encoded by the POMC gene (Gene ID: 5443) in human. POMC is a prohormone that gives rise to several biologically active peptides that are expressed primarily in the pituitary and brain. It is synthesized with a signal peptide (aa 1-26), which is subsequently cleaved off to produce the mature form that is further proteolytically cleaved into eleven different peptides, including NPP (aa 27-102), -MSH (aa 77-87), -MSH (aa 138-150), Corticotropin (ACTH; aa 138-176), β-MSH (aa 217-234), Met-enkephalin (aa 237-241), and β-Endorphin (aa 237-267). POMC-derived peptides are also found in immune cells and are well conserved during evolution. In nervous system POMC neurons are an integral part of the central melanocortin system that regulates feeding behavior. Mutations in POMC gene have been linked to obesity and early-onset obesity with adrenal deficiency. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Millington, GWM. (2007). Nutr. Metab. 4; Article 18; Machelska, H., et al. (2002). Anesthesia & Analgesia. 95(4); 1002-1008).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

30 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.