ZRB1697

Anti-MICA Antibody, clone 4F8 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): MHC class I polypeptide-related sequence A, MIC-A
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• clone: recombinant monoclonal
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 42.92 kDa; observed mol wt ~58 kDa
• species reactivity: rat, mouse, human
• packaging: antibody small pack of 25 μL
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• technique(s): affinity binding assay: suitable; flow cytometry: suitable; immunocytochemistry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• isotype: IgG
• UniProt accession no.: Q29983
• greener alternative category: , Aligned
• shipped in: ambient
• storage temp.: 2-8°C
• target post-translational modification: unmodified

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

Specificity

Clone 4F8 is a ZooMAb rabbit recombinant monoclonal antibody that detects MHC class I polypeptide-related sequence A (MICA). It targets an epitope within the extracellular domain.

Immunogen

KLH-conjugated linear peptide corresponding to 17 amino acids from the extracellular domain of human MHC class I polypeptide-related sequence A (MICA).

Application

Quality Control Testing
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected MICA in NIH3T3 cell lysate.Tested applicationsWestern Blotting Analysis: A 1:1,000 dilution from a representative lot detected MICA in HeLa and L6 cell lysates.Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected MICA in K562 cells.Flow Cytometry Analysis: 1 μg from a representative lot detected MICA in one million K562 cells.Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected MICA in human pancreas and human kidney tissue sections.Affinity Binding Assay: A representative lot of this antibody bound MICA peptide with a KD of 1.5 x 10-6 in an affinity binding assay.Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-MICA, clone 4F8 ZooMAb, Cat. No. ZRB1697, is a recombinant Rabbit monoclonal antibody that targets in MICA and is tested for use in Affinity Binding Assay, Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Target description

MHC class I polypeptide-related sequence A (UniProt: Q29983; also known as MIC-A) is encoded by the MICA (also known as PERB11.1) gene (Gene ID: 100507436) in human. MIC-A is a single-pass type I membrane protein that act as stress-induced self-antigen that are recognized by gamma-delta T cells and serves as ligands for the KLRK1/NKG2D receptor. It is recognized by cytotoxic gamma-delta T cells expressing the TCR variable region V delta1. It is expressed on the cell surface in gastric epithelium, endothelial cells, and fibroblasts and in the cytoplasm in keratinocytes and monocytes. Its expression has also been reported in tumor cells. It is proteolytically cleaved and released from the cell surface of tumor cells which impairs KLRK1/NKG2D expression and T-cell activation. MIC-A can be induced by heat shock and by exposure to DNA damaging conditions. It is synthesized with a signal peptide (aa 1-23), which is cleaved off in the mature form. Their mature forms contain an extracellular domain (aa 24-307), a transmembrane domain (aa 308-328), and a cytoplasmic domain (aa 329-383). Two isoforms of MIC-A have been described that are produced by alternative splicing. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Groh, V., et al. (1999). Proc. Natl. Acad. Sci USA. 96 (12); 6879-6884).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable