ZRB1563

Anti-Caspase-1 p10 Antibody, clone 9H11 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): CASP-1, Caspase-1, EC:3.4.22.36, ICE, IL-1 beta-converting enzyme, IL-1BC, Interleukin-1 beta convertase, Interleukin-1 beta-converting enzyme, p45
• UNSPSC Code: 12352203
• NACRES: NA.43

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 9H11, monoclonal; recombinant monoclonal
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 45.16 kDa (for whole Caspase-1. ); observed mol wt ~10 kDa
• species reactivity: human, mouse
• packaging: antibody small pack of 25 μL
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• technique(s): affinity binding assay: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• isotype: IgG
• UniProt accession no.: P29466
• greener alternative category: , Aligned
• shipped in: ambient
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... CASP1(834) ; mouse ... Casp1(12362)

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

Specificity

Clone 9H11 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects p10 subunit of Caspase-1. It targets an epitope within 17 amino acids from the C-terminal region of the p10 subunit of caspase-1.

Immunogen

KLH-conjugated linear peptide corresponding to 17 amino acids from the C-terminal region of p10 subunit of human Caspase-1.

Application

Anti-Caspase-1 p10, clone 9H11 ZooMAb, Cat. No. ZRB1563, is a Rabbit monoclonal antibody that specifically targets Caspase-1 p10 and is tested for use in Affinity Binding Assay, Immunohistochemistry (Paraffin), and Western Blotting.

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Caspase-1 p10 in lysates from L929 and THP-1 cells treated with Lipopolysaccharide (LPS).

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Caspase-1 p10 in human tonsil tissue sections.

Affinity Binding Assay: A representative lot of this antibody bound Caspase-1 p10 peptide with a KD of 2.8 x 10-9 in an affinity binding assay.



Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Target description

Caspase-1 (UniProt: P29466; also known as EC:3.4.22.36; CASP-1, Interleukin-1 beta convertase, IL-1BC, Interleukin-1 beta-converting enzyme, ICE, IL-1 beta-converting enzyme, p45) is encoded by the CASP1 (also known as IL1BC, IL1BCE) gene (Gene ID: 834) in human. Caspases are secreted as latent single chain zymogens, which are further processed to create active caspases. Caspases are secreted as latent single chain zymogens, which are further processed to create active caspases. The zymogen form of caspase-1 has an N-terminal propeptide (aa 1-119) and another propeptide (aa 298-316), which are cleaved off to generate p20 (aa 120-297) and p10 (aa 317-404) subunits. Its CARD domain is localized in amino acids 1-91. The two subunits (p20 an p10) heterotetramerize to form two anti-parallel arranged heterodimers. Caspase-1 is a thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine that is involved in a variety of inflammatory processes. It is a component of the inflammasome protein complex. It has a preferred cleavage site of Tyr-Val-Ala-Asp (YVAD). This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable