SRP2093

SV40 T-antigen from simian virus

recombinant, expressed in insect cells, ≥80% (SDS-PAGE)

• UNSPSC Code: 12352202

Properties

• biological source: simian virus
• recombinant: expressed in insect cells
• Assay: ≥80% (SDS-PAGE)
• form: frozen liquid
• mol wt: ~83.3 kDa
• packaging: pkg of 5 μg
• storage condition: avoid repeated freeze/thaw cycles
• concentration: 400 μg/mL
• color: clear colorless
• NCBI accession no.: AAG39214.1
• UniProt accession no.: P03070
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: simian virus ... SV40gp6(1489531)

Description

Biochem/physiol Actions

The large T antigen (TAg) of Simian virus 40 acts as an initiator of DNA replication, participates in cellular transformation, and induces cell growth. A number of functional domains have been characterized including the N-terminal J domain, nuclear localization signal (NLS), internal helicase domain, Rb and topoisomerase I interacting domain, p53 and ATP binding domain. The p53-interacting domain is also essential for binding p300/CBP. Phosphorylation of TAg by several kinases may regulate its function both positively and negatively.

The viral replication of simian virus 40 (SV40) is carried out by a single protein, T-antigen. This feature makes the virus a useful system for understanding the process of replication and to identify the components of replication machinery. It functions as a DNA helicase along with a cellular single-strand DNA binding protein and topoisomerase I or II, leading to extensive origin unwinding and formation of a pre-initiation complex. The T-antigen associates with two tumor suppressor proteins, p53 and the retinoblastoma protein pRb, and inactivates them.

Physical form

Clear and colorless frozen liquid solution

Preparation Note

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable