SBR00049

FITC Labeled D-Alanine

Suitable for bacterial labeling

• Empirical Formula (Hill Notation): C₂₄H₁₉N₃O₇S
• Molecular Weight: 493.49
• UNSPSC Code: 12352209
• NACRES: NA.24

Properties

• Quality Level: 200
• form: solid
• storage temp.: −20°C

Description

General description

Bacterial Peptidoglycan is a polymer consisting of sugars and amino acids, a mesh-like rigid layer that forms the cell wall. D-Amino Acids (D-AAs) are metabolically incorporated onto the bacterial cell wall by D,D-transpeptidase and/or L,D-transpeptidase. It was recently discovered that those transpeptidases catalyze the metabolic incorporation of exogenous D-AAs with almost no restriction of the side-chain identity. Modification of a D-AA with molecular fluorophores provides fluorescent d-amino acids that can efficiently label in situ peptidoglycan in diverse bacterial species.
Fluorescein Labeled D-Alanine is a fluorescent derivative of D-alanine that is obtained by a synthetic conjugation of 3-amino-D-alanine to a fluorophore. Fluorescein Labeled D-Alanine has high biocompatibility and suitability for labeling peptidoglycans in live bacteria

Application

Fluorescent labeled D-AAs can be used for many applications including:

• Bacterial cell wall morphology
• Bacterial cell wall formation or remodeling activity
• Bacterial viability/activity
• Identify bacterial activity on surfaces or in substances

Analysis Note

• Fluorescent microscopy application: Fluorescein Labeled D-Alanine has excitation/emission wavelength range at 495 nm/515 nm.
• The recommended working concentration in fluorescent microscopy imaging application is between 250 μM-500 μM in DMSO.
• Aliquots of the DMSO solution can be stored at -20°C, protected from light for at least one month.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable