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Key Documents

SAB4503371

Sigma-Aldrich

Anti-NT5C1A antibody produced in rabbit

affinity isolated antibody

Synonym(s):

Cytosolic 5′-nucleotidase 1A, Cytosolic 5′-nucleotidase IA, cN-I, cN-IA, cN1A

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 41 kDa

species reactivity

mouse, human

concentration

~1 mg/mL

technique(s)

ELISA: 1:20000
immunofluorescence: 1:100-1:500
western blot: 1:500-1:1000

NCBI accession no.

Q9BXI3

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... 5NT1A(84618)

Related Categories

General description

Anti-NT5C1A Antibody detects endogenous levels of total NT5C1A protein. The NT5C1A (5′-nucleotidase, cytosolic IA) gene is mapped to human chromosome 1p34. The protein encoded is ubiquitously expressed and is localized to the cytoplasm.

Immunogen

The antiserum was produced against synthesized peptide derived from human NT5C1A.

Immunogen Range: 151-200

Biochem/physiol Actions

NT5C1A (5′-nucleotidase, cytosolic IA) forms a tetramer with AMP (adenosine monophosphate) and serves as a primary substrate in adenosine pathway to control the level of metabolite produced. NT5C1A catalyzes the dephosphorylation of AMP to adenosine.

Features and Benefits

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Physical form

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

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Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Suppression of 5'nucleotidase enzymes promotes AMPK phosphorylation and metabolism in human and mouse skeletal muscle.
Kulkarni S S, et al.
The Journal of Biological Chemistry (2011)
Pharmacogenetic characterization of naturally occurring germline NT5C1A variants to chemotherapeutic nucleoside analogs.
Saliba J, et al.
Pharmacogenetics and Genomics, 26(6), 271-271 (2016)
Expression of cytosolic 5? nucleotidase does not correlate with expression of oxidative metabolism marker: myoglobine in human skeletal muscles.
Lechward K and Tkacz-Stachowska K
Acta biochimica et biophysica Sinica, 41(4), 280-284 (2009)

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