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Key Documents

N2147

Sigma-Aldrich

Nutrient Mixture F-10 Ham

With HEPES, without sodium bicarbonate and ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

Ham’s F-10

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About This Item

MDL number:
UNSPSC Code:
12352207
NACRES:
NA.75

product name

Nutrient Mixture F-10 Ham, With 20 mM HEPES, without sodium bicarbonate and L-glutamine, liquid, sterile-filtered, suitable for cell culture

Quality Level

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

pH

>7.2

components

HEPES: 20 mM
L-glutamine: no
sodium pyruvate: 0.11 g/L
phenol red: yes
NaHCO3: no

shipped in

ambient

storage temp.

2-8°C

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Reconstitution

Supplement with 0.146 g/L L-glutamine.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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Xiukun Wang et al.
Development (Cambridge, England), 146(13) (2019-06-14)
Epigenetic regulation, including histone-to-protamine exchanges, controls spermiogenesis. However, the underlying mechanisms of this regulation are largely unknown. Here, we report that PHF7, a testis-specific PHD and RING finger domain-containing protein, is essential for histone-to-protamine exchange in mice. PHF7 is specifically

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