MSKT0003

IA-MS Immunoaffinity Mass Spectrometry Kit - Streptavidin

96 Well Strip Plate with SOLu-Trypsin

• Synonym(s): Target protein enrichment and quantification from complex background by mass spectrometry
• UNSPSC Code: 41115859
• NACRES: NA.41

Properties

• form: solid
• suitability: suitable for mass spectrometry
• shipped in: wet ice
• storage temp.: 2-8°C

Description

General description

The Streptavidin IA-MS Immunoaffinity Mass Spectrometry Kit is a 96-well platform enabling robust, high-throughput quantification of proteins in complex backgrounds by LC-MS/MS. Users provide their own biotinylated capture molecule and internal standard (available separately, internal standards may be SIL peptides or preferably full length SIL protein) and the kit contains all other components needed for in-well enrichment and rapid trypsin digestion of samples. The plate format consists of 8-well strips allowing for partial runs, and avoids the high cost and potential LC column blockage associated with bead-based enrichment formats. The entire workflow can be performed in under 5 hours, resulting in digests ready for injection with no SPE or other cleanup required.

Application

Target protein enrichment and quantification from complex background by mass spectrometry.

Features and Benefits

• Entire workflow including digest completed in under 5 hours, such that samples are injection ready at the end of the work day
• 96-well plate format has potential for automation in a liquid-handling system
• Bead-free system circumvents messy clean-up and eliminates risk of column clogging
• Plate strips allow user to run as many or as few samples as desired

Packaging

Box with foam insert containing kit components.

Components

Streptavidin High Binding Capacity Coated Plate S2577-1EA
SOLu-Trypsin EMS0004-100UG
MS Denaturation Solution EMS00010-10ML
Rapid Trypsin Digestion Buffer EMS0009-30ML
Tris Buffered Saline with TWEEN^® 20 (TBST)powder, pH 8.0 T9039
Tris Buffered Saline (TBS) powder, pH 8.0 T6664
EZ-Pierce^™ plate seal Z721581-1EA

See datasheet for more information.

Storage and Stability

2 years when stored at 2-8 °C.

Related Presentation

• Development of a Plate-based Immunoaffinity LC-MS/MS Workflow for Sensitive Quantitation of Free Infliximab and Adalimumab in Human Serum (Poster, PDF)

Legal Information

EZ-Pierce is a trademark of Excel Scientific, Inc.

TWEEN is a registered trademark of Croda International PLC

Kit Components Only

• Rapid Trypsin Digestion Buffer 30 mL
• MS Denaturation Solution

Kit Components Also Available Separately

• S2577 Streptavidin High Capacity Coated Plates, 8 well strip, clear 1 plate
• EMS0004 SOLu-Trypsin, recombinant, expressed in Pichia pastoris, Proteomics Grade, liquid 4 ´ 100 mL (1 mg/mL)
• S2577 Streptavidin High Capacity Coated Plates, 8 well strip, clear 1 packet
• T6664 Tris Buffered Saline, pH 8.0, powder
• Z721581 EZ-Pierce^™ films, Excel Scientific EZP-100, non-sterile 4 films

Safety Information

• Pictograms: GHS02,GHS06,GHS08
• Signal Word: Danger
• Hazard Statements: H226 - H301 + H311 + H331 - H370
• Precautionary Statements: P210 - P233 - P280 - P301 + P310 - P303 + P361 + P353 - P304 + P340 + P311
• Hazard Classifications: Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Flam. Liq. 3 - STOT SE 1
• Storage Class Code: 3 - Flammable liquids
• WGK: WGK 3
• Flash Point(F): 75.2 °F
• Flash Point(C): 24 °C