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MAK179

Sigma-Aldrich

Oxalic Acid Colorimetric Assay Kit

Sufficient for 100 Colorimetric tests

Synonym(s):

Oxalate Assay Kit

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About This Item

EC Number:
UNSPSC Code:
12161503
NACRES:
NA.84

detection method

colorimetric

relevant disease(s)

genitourinary disorders

storage temp.

−20°C

General description

Oxalic acid (Oxalate) is a strong dicarboxylic acid that occurs in several plants and vegetables. In animals, it is either incorporated from the diet or produced during ascorbic acid and glyoxylic acid metabolism. Oxalic acid is not metabolized and is excreted in the urine. Hence, it can act as a marker for glyoxylic and ascorbic acid metabolism. Furthermore, oxalic acid has been studied as a pathogenic factor for Sclerotinia sclerotiorum that inhibits the oxidative burst response in host plants. It is also known to alleviate chilling injury in peach fruit. Elevated levels of oxalic acid (oxalate) have been associated with the formation of kidney stones. Hence, oxalate measurements can be used for the diagnosis and monitoring of renal stones.

Application

Oxalic acid colorimetric assay kit has been used in 24-hour urinary oxalate level (24HUOL) evaluation in an animal model.

Suitability

Suitable for the detection of oxalate (oxalic acid) in biological samples including animal and plant tissue.

Principle

The Oxalic Acid Assay Kit provides a simple and sensitive procedure for measuring oxalic acid in variety of samples including animal and plant tissue. In this assay, oxalic acid concentration is determined by a coupled enzyme reaction, which results in a colorimetric (450 nm) product, proportional to the oxalate present.

Kit Components Only

Product No.
Description

  • Oxalate Assay Buffer

  • Oxalate Developer Buffer

  • Oxalate Converter

  • Oxalate Enzyme Mix

  • Oxalate Probe

  • Oxalate Standard

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Oral

Storage Class Code

10 - Combustible liquids


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Oxalic acid excretion after intravenous ascorbic acid administration
Line R, et al.
Metabolism, Clinical and Experimental, 58(2) , 263-269 (2009)
A structural investigation of the interaction of oxalic acid with Cu(110)
White T W, et al.
Surface Science, 668, 134-143 (2018)
Strategies for preventing calcium oxalate stones
Vadim A F, et al.
CMAJ : Canadian Medical Association Journal = Journal de l'Association Medicale Canadienne, 174(10), 1407-1409 (2006)
Oxalic Acid, a Pathogenicity Factor for Sclerotinia sclerotiorum, Suppresses the Oxidative Burst of the Host Plant.
Stephen G C, et al.
Plant Cell, 12(11), 2191?2200-2191?2200 (2000)
Characterizing the Association Between Toll-like Receptor Subtypes and Nephrolithiasis With Renal Inflammation in an Animal Model.
Olcucu M T, et al.
Urology, 111 , 238-e1-238-e1 (2018)

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