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MAK165

Sigma-Aldrich

Fluorimetric Hydrogen Peroxide Assay Kit

sufficient for 500 fluorometric tests (red fluorescence)

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About This Item

UNSPSC Code:
12161503

usage

sufficient for 500 fluorometric tests (red fluorescence)

detection method

fluorometric

relevant disease(s)

cardiovascular diseases; aging/geriatric diseases; orthopedic diseases; pulmonary disorders; neurological disorders

storage temp.

−20°C

Related Categories

General description

Hydrogen peroxide, a reactive oxygen species produced through the metabolism of molecular oxygen, serves as both an intracellular signaling messenger and a source of oxidative stress. Hydrogen peroxide is generated in cells via multiple mechanisms such as the NOX-mediated ROS production by neutrophils and macrophages (respiratory burst) or by the dismutase of superoxide anions produced as a result of electron leak during mitochondrial respiration. Abnormal hydrogen peroxide production contributes to oxidative cell damage and the progression of diseases such as asthma, atherosclerosis, osteoporosis, and neurodegeneration.

Features and Benefits

Compatible with high-throughput handling systems.

Suitability

This kit is suitable to quantify hydrogen peroxide levels in a variety of samples such as cellular extracts and solutions.

Principle

The Fluorescent Hydrogen Peroxide Assay Kit provides a simple and reproducible method to quantify hydrogen peroxide levels in a variety of samples such as cellular extracts and solutions. This kit can also be used to detect hydrogen peroxide released from living cells or produced by oxidase activities. This kit utilizes a peroxidase substrate that generates a red fluorescent product (λex = 540/λem = 590 nm) after reaction with hydrogen peroxide that can be analyzed by a fluorescent microplate reader.

Pictograms

Exclamation markHealth hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Acute Tox. 4 Oral - Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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Foteini Vasilopoulou et al.
GeroScience, 43(2), 965-983 (2020-11-01)
Brain aging and dementia are current problems that must be solved. The levels of imidazoline 2 receptors (I2-IRs) are increased in the brain in Alzheimer's disease (AD) and other neurodegenerative diseases. We tested the action of the specific and selective
Wei Zhu et al.
Oxidative medicine and cellular longevity, 2019, 5305014-5305014 (2019-06-11)
Females develop kidney stones less frequently than males do. However, it is unclear if this gender difference is related to altered estrogen/estrogen receptor (ER) signaling. Here, we found that ER beta (ERβ) signals could suppress hepatic oxalate biosynthesis via transcriptional
Yan Zhang et al.
Journal of nanobiotechnology, 19(1), 161-161 (2021-06-02)
As one typical cardiovascular disease, atherosclerosis severely endanger people' life and cause burden to people health and mentality. It has been extensively accepted that oxidative stress and inflammation closely correlate with the evolution of atherosclerotic plaques, and they directly participate
Abraham Lin et al.
Oxidative medicine and cellular longevity, 2020, 9750206-9750206 (2020-12-22)
Non-thermal plasma (NTP), an ionized gas generated at ambient pressure and temperature, has been an emerging technology for medical applications. Through controlled delivery of reactive oxygen and nitrogen species (ROS/RNS), NTP can elicit hormetic cellular responses, thus stimulating broad therapeutic
Rakesh Ruchel Khanikar et al.
RSC advances, 12(15), 9466-9472 (2022-04-16)
Cold atmospheric pressure (CAP) plasma has a profound effect on protein-protein interactions. In this work, we have highlighted the deactivation of the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) spike protein by CAP plasma treatment. Complete deactivation of spike protein

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