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L0144

Sigma-Aldrich

Lucifer Yellow CH dipotassium salt

powder

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About This Item

Empirical Formula (Hill Notation):
C13H9K2N5O9S2
CAS Number:
Molecular Weight:
521.57
Beilstein:
5224942
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.77

form

powder

color

orange

fluorescence

λex 428 nm; λem 540 nm

storage temp.

room temp

SMILES string

[K+].[K+].NNC(=O)NN1C(=O)c2cc(cc3c(N)c(cc(C1=O)c23)S([O-])(=O)=O)S([O-])(=O)=O

InChI

1S/C13H11N5O9S2.2K/c14-10-5-1-4(28(22,23)24)2-6-9(5)7(3-8(10)29(25,26)27)12(20)18(11(6)19)17-13(21)16-15;;/h1-3H,14-15H2,(H2,16,17,21)(H,22,23,24)(H,25,26,27);;/q;2*+1/p-2

InChI key

HCWYSCVNSCIZCN-UHFFFAOYSA-L

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General description

Lucifer yellow CH is a fixable dye with an excitation and emission spectrum of 430 nm and 540 nm, respectively.LuciferYellow CH (LYCH) is a small, hydrophilic molecule, and highly fluorescent dye that is used for intracellular staining for the studies conducted on various types of cells. It acts as a marker for the detection of well-established tight junctions in smooth muscles, glia, and interstitial cells of Cajal (ICC).

Application

Lucifer yellow CH dipotassium salt has been used:
  • to visualize cell morphology
  • to assess intercellular communication activity through gap junction by scrape-loading assay
  • as a component of the intracellular solution, which filled the dendrites for the visualization of neuronal morphology
  • in the intracellular solution, to intracellularly label recorded neurons

Features and Benefits

Highly fluorescent dye, useful in marking nerve cells.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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The morphology and intrinsic excitability of developing mouse retinal ganglion cells
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