I2526

Insulin-like Growth Factor-II human

IGF-II, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• Synonym(s): IGF-II
• MDL number: MFCD00241895
• UNSPSC Code: 12352202
• NACRES: NA.77

Properties

• biological source: human
• Quality Level: 200
• recombinant: expressed in E. coli
• Assay: ≥98% (SDS-PAGE and HPLC)
• form: lyophilized powder
• potency: ≤2.0 ng/mL ED₅₀
• quality: endotoxin tested
• mol wt: 7.5 kDa
• packaging: pkg of 5X50 μg; pkg of 50 μg
• technique(s): cell culture | mammalian: suitable
• impurities: ≤0.1 EU/μg
• UniProt accession no.: P01344
• storage temp.: −20°C
• Gene Information: human ... IGF2(3481)

Description

Biochem/physiol Actions

Insulin-like growth factor II (IGF-II) also known as multiplication stimulating activity (MSA), shows virtually identical bioactivities as IGF-I. Expression of IGF-II is highest in fetal tissues, where it is believed to play a major role in overall growth and development. After birth IGF-II expression decreases to much lower levels in a variety of tissues. IGF-II is produced also in several tumors. IGF-II binds to IGF-II receptor (IGF-IIR), to IGF-IR and weakly to the insulin receptor (IR). All IGF-II bioactivities in adult tissues are generally attributed to its interaction with IGF-IR. However, during embryogenesis the roles of both IGF-IIR and IR may be significant in promoting normal growth.

Physical form

Lyophilized from a 0.2 μm filtered solution with no additives.

Analysis Note

The biological activity is measured by a cell proliferation assay using mouse FDC-P1

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves