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H9914

Millipore

HIS-Select® Nickel Magnetic Agarose Beads

Synonym(s):

nickel charged magnetic beaded agarose

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About This Item

UNSPSC Code:
41106500
NACRES:
NA.56

conjugate

magnetic beads

form

suspension

shelf life

2 yr (Unopened product)

matrix

6% beaded magnetic agarose

capacity

≥15 mg/mL binding capacity

storage temp.

2-8°C

General description

HIS-Select Magnetic Agarose Beads consist of paramagnetic, immobilized metal-ion affinity chromatography (IMAC) resin that contain a proprietary quadridentate chelate, which is bound with nickel and covalently attached through a non-charged, hydrophilic linker to magnetic beaded agarose. The magnetic properties of the beads aid in manipulations, such as repetitive washings, and recovery of the protein bound beads. This leads to greater experimental reproducibility and more accurate quantitation of the His-tagged proteins of interest.

Application

The HIS-Select Nickel Magnetic Agarose Beads are designed for use in automated and small-scale affinity capture (molecular pull-down) purifications of histidine-tagged protein / His-tag protein while exhibiting low non-specific binding of other proteins. The beads can be used to purify His-tagged proteins under native and denaturing conditions.

Physical form

Supplied as a 50% slurry suspension in 30% Ethanol.

Legal Information

HIS-Select is a registered trademark of Merck KGaA, Darmstadt, Germany

Signal Word

Danger

Hazard Classifications

Aquatic Chronic 3 - Carc. 1B - Flam. Liq. 3 - Repr. 1B - Skin Sens. 1 - STOT RE 2

Target Organs

Respiratory Tract

Storage Class Code

3 - Flammable liquids

WGK

WGK 3

Flash Point(F)

86.0 °F

Flash Point(C)

30 °C

Certificates of Analysis (COA)

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Sonja Hänzelmann et al.
Clinical epigenetics, 7, 19-19 (2015-03-13)
Primary cells enter replicative senescence after a limited number of cell divisions. This process needs to be considered in cell culture experiments, and it is particularly important for regenerative medicine. Replicative senescence is associated with reproducible changes in DNA methylation
Mei Suen Kong et al.
Science signaling, 12(567) (2019-02-07)
T cell activation is initiated by signaling molecules downstream of the T cell receptor (TCR) that are organized by adaptor proteins. CIN85 (Cbl-interacting protein of 85 kDa) is one such adaptor protein. Here, we showed that CIN85 limited T cell
Joshua W Burgess et al.
American journal of respiratory cell and molecular biology, 41(6), 714-721 (2009-03-17)
Pneumocystis organisms are opportunistic fungal pathogens that cause significant pneumonia in immune-compromised hosts. Recent evidence has suggested that Pneumocystis carinii exists as separate mating types, and expresses and regulates proteins that govern meiosis and progression of the life cycle. This
Noel J Byrne et al.
Protein expression and purification, 179, 105796-105796 (2020-11-23)
TREM2 has been identified by genomic analysis as a potential and novel target for the treatment of Alzheimer's disease. To enable structure-based screening of potential small molecule therapeutics, we sought to develop a robust crystallization platform for the TREM2 Ig-like
Xing Huang et al.
Autophagy, 15(7), 1258-1279 (2019-02-23)
Notwithstanding the numerous drugs available for liver cancer, emerging evidence suggests that chemotherapeutic resistance is a significant issue. HGF and its receptor MET play critical roles in liver carcinogenesis and metastasis, mainly dependent on the activity of receptor tyrosine kinase.

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