H3166

Anti-HOXD4 (C-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonym(s): Anti-HHO.C13, Anti-HOX-4B, Anti-HOX-5.1
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~35 kDa
• species reactivity: human
• concentration: ~1.0 mg/mL
• technique(s): immunoprecipitation (IP): 1.5-3 μg using lysates of HEK-293T cells over-expressing human HOXD4; indirect immunofluorescence: 0.25-0.5 μg/mL using paraformaldehyde fixed HEK-293T cells over expressing human HOXD4; western blot: 0.5-1 μg/mL using lysates of HEK-293T cells over-expressing human HOXD4
• UniProt accession no.: P09016
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... HOXD4(3233) ; rat ... Hoxd4(288153)

Description

General description

The Homeobox D4 (HOXD4) gene is expressed in mesodermal tissues and the central nervous system (CNS). The HOXD4 gene is mapped on the human chromosome at 2q31.1.

Specificity

Anti-HOXD4 (C-terminal) recognizes human HOXD4 (also known as HOX-4B, HOX-5).

Application

Anti-HOXD4 (C-terminal) antibody produced in rabbit may be used in:

• immunoblotting
• immunoprecipitation
• immunofluorescence

Biochem/physiol Actions

The Homeobox D4 (HOXD4) gene is involved in the development of dura mater and morphogenesis of the development of the spine. This gene plays a role in the pathogenesis of spinal extradural arachnoid cyst (SEDAC). HOXD4 gene is also involved in tumorigenesis of the central nervous system. This gene acts as a diagnostic marker of gliomas.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

Store at –20 °C. For continuous use, the product may be stored at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.