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GE17-5113-01

Streptavidin Sepharose™ High Performance

Cytiva 17-5113-01, pack of 5 mL

Synonym(s):

affinity chromatography medium, high performance affinity resin, biotinylated proteins purification

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About This Item

UNSPSC Code:
41106500
NACRES:
NA.56

packaging

pack of 5 mL

manufacturer/tradename

Cytiva 17-5113-01

storage condition

(20% Ethanol)

matrix

highly cross-linked 6% agarose

avg. part. size

34 μm

cleaning in place

2-10.5

working range

4-9

capacity

binding capacity(>300 nmol biotin/ml medium 6 mg biotinylated BSA/ml medium)

storage temp.

2-8°C

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General description

Streptavidin Sepharose™ High Performance is an affinity chromatography medium designed for fast and reliable, high resolution purification of biotinylated biomolecules.

Purified Streptavidin isolated from Streptomyces avidinii is immobilised on Sepharose™ High Performance. The base matrix is a rigid, highly cross-linked beaded agarose with high chemical stability. The immobilised streptavidin binds biotin and biotinylated substances through affinity chromatography.

Features and Benefits

  • Streptavidin immobilized to Sepharose™ High Performance for purification of biotinylated compounds
  • Extremely useful for exploiting either the strong interactions of biotin and streptavidin or the somewhat weaker interactions of 2-iminobiotin and streptavidin.
  • 34 μm bead size for improved resolution

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Sepharose is a trademark of Cytiva

Pictograms

Flame
GHS02

Signal Word

Warning

Hazard Statements

H226

Storage Class Code

3 - Flammable liquids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Jérôme Zervudacki et al.
The EMBO journal, 37(14) (2018-06-07)
Mobilization of transposable elements (TEs) in plants has been recognized as a driving force of evolution and adaptation, in particular by providing genes with regulatory modules that impact their transcription. In this study, we employed an ATCOPIA93 long-terminal repeat (LTR)
Hana Antonicka et al.
Cell metabolism, 32(3), 479-497 (2020-09-03)
We used BioID, a proximity-dependent biotinylation assay with 100 mitochondrial baits from all mitochondrial sub-compartments, to create a high-resolution human mitochondrial proximity interaction network. We identified 1,465 proteins, producing 15,626 unique high-confidence proximity interactions. Of these, 528 proteins were previously
Sarah van Veen et al.
Nature, 578(7795), 419-424 (2020-01-31)
ATP13A2 (PARK9) is a late endolysosomal transporter that is genetically implicated in a spectrum of neurodegenerative disorders, including Kufor-Rakeb syndrome-a parkinsonism with dementia1-and early-onset Parkinson's disease2. ATP13A2 offers protection against genetic and environmental risk factors of Parkinson's disease, whereas loss

Articles

Affinity Chromatography Troubleshooting

This page shows how to solve practical problems that may occur when running an affinity chromatography column.

Protocols

Purification or Removal of Biotin and Biotinylated Substances

This page shows how to purify or remove biotin and biotinylated substances with Streptavidin Sepharose High Performance from GE Healthcare.

Converting From Linear Fow (cm/hour) to Volumetric Flow Rates (mL/min) and Vice Versa

This page shows how to convert between linear flow and volumetric flow rates in affinity chromatography.

Column Packing and Preparation Protocol for Affinity Chromatography

This page shows how to perform column packing and preparation for affinity chromatography when using Tricorn™ or XK columns available from GE Healthcare.

Sample Preparation for Affinity Chromatography

This page shows how to prepare samples for purification with affinity chromatography.

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