PNT2 Cell Line human
95012613, human prostate, Epithelial
Synonym(s):
PNT-2 Cells
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About This Item
UNSPSC Code:
41106514
product name
PNT2 Cell Line human, Normal prostate epithelium immortalized with SV40., 95012613
biological source
human prostate
growth mode
Adherent
karyotype
Not specified
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Human prostate normal, immortalised with SV40
Cell Line Description
Established by immortalisation of normal adult prostatic epithelial cells by transfection with a plasmid containing SV40 genome with a defective replication origin. The primary culture was obtained from a prostate of a 33 year old male at post mortem. PNT2 cells contain the SV40 genome and express large T protein. They possess a well differentiated morphology with the expression of cytokeratin 8,18 and 19 with the latter being a feature of differentiated luminal cells of the glandular prostate. Cytokeratin 14, a marker of epithelial basal cells, is not expressed. The cells are non-tumourigenic in nude mice.
Application
Biology of prostate cells, model of differentiated prostate epithelial cells.
PNT2 cell line has been used:
- to assess the effects of Verapamil, an L-type calcium channel blocker on autophagy
- to assess the cytotoxicity of aqueous dilutions of a universal self-priming dental adhesive (DA)
- to study the expression of soluble adenylyl cyclase (sAC) in the cells
DNA Profile
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,13
D13S317: 8,13
D16S539: 9
D5S818: 12,13
D7S820: 11
THO1: 6,9.3
TPOX: 8,9
vWA: 17
CSF1PO: 11,13
D13S317: 8,13
D16S539: 9
D5S818: 12,13
D7S820: 11
THO1: 6,9.3
TPOX: 8,9
vWA: 17
Culture Medium
RPMI 1640 + 2mM Glutamine + 6 -10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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Certificates of Analysis (COA)
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