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Key Documents

AFRC MAC 57

86030310, Reactivity: Anti-Lipopolysaccharide

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About This Item

UNSPSC Code:
41106514

biological source

Rat x Rat Hybridoma

ImmunoDon

LOU/Iap rat spleen

Myeloma

Y3Ag1.2.3

Reactivity

Anti-Lipopolysaccharide

technique(s)

cell culture | mammalian: suitable

isotype

IgM

shipped in

dry ice

storage temp.

−196°C

Cell Line Origin

Rat Y3Ag1.2.3 x Rat LOU/Iap spleen

Cell Line Description

The antibody is directed against lipopolysaccharide components of the bacterial cell wall and also with lipopolysaccharide from free-living cultures of Rhizobium strains 3841 and B155.

Immunogen

Sonicated bacteroid material from Rhizobium leguminosarum strain 3841 and strain B155

Culture Medium

DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)

Subculture Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. These cells require mouse macrophage feeder layers upon resuscitation

Other Notes

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