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biological source
mouse skin
growth mode
Suspension
karyotype
Aneuploid
morphology
Lymphoblast
products
Immunoglobulin G (IgG1) (kappa)
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Related Categories
Cell Line Origin
Mouse myeloma
Cell Line Description
Derived from the P3X27 cell line, itself a derivative of mineral oil-induced plasmacytoma MOPC-21 in BALB/c mice. Resistant to 20μg/ml azaguanine, does not grow in selective medium. HGPRT neg., dies in HAT. Doubling time 16-26 h.
Application
Fusion partner
Culture Medium
DMEM or RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Maintain cultures between 5x10,000 to 3x100,000 cells/ml; 5% CO2; 37°C. Cells will achieve maximum cell density of 5x100,000 cells/ml.
Other Notes
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Certificates of Analysis (COA)
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