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product name
LA1-55n, 06041203
biological source
human nerve
growth mode
Semi-adherent aggregates
karyotype
Not specified
morphology
Fusiform cells
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Human Neuroblastoma
Cell Line Description
Derived from LA1-5s a clonal subline of LA-N-1. LA-N-1 was derived from neuroblastoma cells in the bone marrow of a 2-year-old male with clinical Stage IV neuroblastoma.LA-N-1 (Sigma Catalogue number 06041201), LA1-55n (Sigma Catalogue number 06041203) and LA1-5s (Sigma Catalogue number 06041204) have been shown to originate from the same patient by STR profiling.
Culture Medium
EMEM (with non-essential amino acids) and Ham′s F12 (1:1 mixture) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)
Subculture Routine
For routine maintenance, split cultures only after they have become very dense i.e. split once every 2-3 weeks at a 1:20 to 1:100 ratio; 8% CO2, 37°C. Cells are moderate to weakly adherent. Allow floating clumps to settle then withdraw medium. Remove at
Other Notes
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Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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