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C6788

Sigma-Aldrich

4-Chloro-1-naphthol

peroxidase substrate, tablet, suitable for use in immunoblotting procedures

Synonym(s):

4-CN, 4-chloronaphthalen-1-ol

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About This Item

Linear Formula:
ClC10H6OH
CAS Number:
604-44-4
Molecular Weight:
178.61
Beilstein:
509750
EC Number:
210-068-5
MDL number:
MFCD00003974
UNSPSC Code:
12352204
PubChem Substance ID:
57654035
NACRES:
NA.83

product name

4-Chloro-1-naphthol, tablet

form

tablet

mp

118-121 °C (lit.)

solubility

methanol: 10 mL, clear, colorless

storage temp.

−20°C

SMILES string

Oc1ccc(Cl)c2ccccc12

InChI

1S/C10H7ClO/c11-9-5-6-10(12)8-4-2-1-3-7(8)9/h1-6,12H

InChI key

LVSPDZAGCBEQAV-UHFFFAOYSA-N

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Specificity

4-Chloro-1-Naphthol is a peroxidase substrate suitable for use in immunoblotting procedures. This substrate produces an insoluble end product that is blue in color and can be observed visually.

Application

4-Chloro-1-naphthol has been used as a substrate in enzyme-linked immunospot (ELISpot) assay for detection of the spots. It has also been used as a precipitating chromogenic substrate for visualizing the antigen−antibody-reactive bands following western blotting.

Quantity

Contains 30 mg substrate per tablet.

Reconstitution

Dissolve 1 tablet in 10 ml of methanol. Add 2 ml of methanol stock solution to 10 ml of triethanolamine buffered saline, pH 7.5. Add 5 μl of fresh 30% hydrogen peroxide (Sigma Product No. H-1009) immediately prior to use.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Precolostral serology in calves born from Neospora-seropositive mothers
Staubli D, et al.
Parasitology Research, 99(4), 398-404 (2006)
Daniela Staubli et al.
Parasitology research, 99(6), 648-658 (2006-05-24)
Neospora caninum ranges among the major causes of infectious abortion in cattle worldwide. The present study was designed to improve the serodiagnostic tools by complementing a conventional ELISA with a highly sensitive and species-specific N. caninum immunoblot. To evaluate this
Karl Albert Brokstad et al.
The Journal of infectious diseases, 185(7), 878-884 (2002-03-29)
The immune response in the nasal mucosa to influenza vaccination in 23 patients scheduled for tonsillectomy was studied. A statistically significant increase in influenza virus-specific serum and oral fluid antibodies was observed 7 days after vaccination. The numbers of influenza
H Ellakany et al.
Avian pathology : journal of the W.V.P.A, 27(6), 547-554 (2008-05-17)
Two experiments were conducted to test the sensitivity of Western blotting for detection of M. gallisepticum antibodies in respiratory washings and sera of infected chickens by mouse monoclonal antibodies to chicken IgG, IgM and IgA. In the first experiment, birds
C F Frey et al.
Parasitology research, 104(6), 1269-1277 (2009-01-09)
Trichinellosis is a zoonotic disease in humans caused by Trichinella spp. According to international regulations and guidelines, serological surveillance can be used to demonstrate the absence of Trichinella spp. in a defined domestic pig population. Most enzyme-linked immunosorbent assay (ELISA)
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Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

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