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A9099

Sigma-Aldrich

Acrylamide

for molecular biology, ≥99% (HPLC)

Synonym(s):

2-Propenamide, Polyacrylamide, acrylic amide, akrylamid, Acrylic acid amide

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About This Item

Linear Formula:
CH2=CHCONH2
CAS Number:
Molecular Weight:
71.08
Beilstein:
605349
EC Number:
MDL number:
UNSPSC Code:
41105319
eCl@ss:
39031205
PubChem Substance ID:
NACRES:
NA.31

grade

for molecular biology

Quality Level

vapor density

2.45 (vs air)

vapor pressure

0.03 mmHg ( 40 °C)

Assay

≥99% (HPLC)

form

crystals

impurities

≤0.005% Acrylic acid content
≤0.005% Water insolubles

conductivity

≤10 μmho (35% solution)

bp

125 °C/25 mmHg (lit.)

mp

82-86 °C (lit.)

solubility

water: soluble 2.5 g/10 mL, clear, colorless

cation traces

Fe: ≤1 ppm
Mg: ≤3 ppm
heavy metals (as Pb): ≤5 ppm

foreign activity

DNase, RNase, protease, none detected

SMILES string

NC(=O)C=C

InChI

1S/C3H5NO/c1-2-3(4)5/h2H,1H2,(H2,4,5)

InChI key

HRPVXLWXLXDGHG-UHFFFAOYSA-N

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General description

Polyacrylamide gels are prepared using acrylamide and bis-acrylamide. Acrylamide is a water-soluble monomer, which forms a transparent stable insoluble gel. Bis-acrylamide is a cross-linking agent.

Application

Acrylamide has been used:
  • in polyacrylamide gel electrophoresis
  • in the preparation of cleared gel samples
  • to determine the effect of L-cysteine on acrylamide-induced testicular toxicity

Pictograms

Skull and crossbonesHealth hazard

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Carc. 1B - Eye Irrit. 2 - Muta. 1B - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 1 Oral

Target Organs

Peripheral nervous system

Storage Class Code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Flash Point(F)

280.4 °F - closed cup

Flash Point(C)

138 °C - closed cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

EU REACH SVHC Candidate List

CAS No.

EU REACH Annex XVII (Restriction List)

CAS No.

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Ana Techniqs in Biotechnology (2011)
Mild myelin disruption elicits early alteration in behavior and proliferation in the subventricular zone.
Gould EA,et al.
eLife, 7(4-5), e34783-e34783 (2018)
Separation of Thylakoid Protein Complexes with Two-dimensional Native-PAGE.
Rantala M, et al.
The Plant Journal, 8(13), 29089-29089 (2017)
Electrical characterization of DNA supported on nitrocellulose membranes.
Al AM, et al.
Scientific Reports, 6(4), 29089-29089 (2016)
L-Cysteine Partially Protects Against Acrylamide-Induced Testicular Toxicity.
Kacar S, et al.
Balkan medical journal, 35(4), 311-311 (2018)

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