A0705
Anti-Bovine IgG (whole molecule)−Alkaline Phosphatase antibody produced in rabbit
affinity isolated antibody
Synonym(s):
Rabbit Anti-Bovine IgG (whole molecule)−AP
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About This Item
biological source
rabbit
conjugate
alkaline phosphatase conjugate
antibody form
affinity isolated antibody
antibody product type
secondary antibodies
clone
polyclonal
species reactivity
bovine
technique(s)
direct ELISA: 1:30,000
dot blot: 1:30,000
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
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General description
Immunoglobulins (Igs) belongs to immunoglobulin super-family (IgSF). Igs in general, consist of two heavy (H) chains and two light (L) chains. The L chain can be either κ or a λ chain. Each chain contains one NH2-terminal “variable" (V) region and one or more COOH-terminal “constant” (C) regions. Each of which consists of two sandwiched β pleated sheets ′pinned′ together by a disulfide bridge. There are five main classes of Igs based on heavy chain C domains, they are, IgM, IgG, IgA, IgD, and IgE isotypes. IgG can be divided into four subclasses, IgG1, IgG2, IgG3, and IgG4. Each subclass possesses distinct biologic properties.
Immunogen
Purified bovine IgG
Application
Anti-Bovine IgG (whole molecule)-Alkaline Phosphatase antibody produced in rabbit has been used in
- enzyme linked immunosorbent assay (ELISA)
- development of immunosensors
- western blot analysis
Biochem/physiol Actions
Bovine IgGs are glycoprotein antibodies that regulate immune responses in herds. These antibodies inhibit TLR5 activation upon immunization with native H7 flagellin . Bovine IgG levels can be used for the detection of Johne′s disease and milk adulteration .
Physical form
Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2, 50% glycerol, and 15 mM sodium azide
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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Carla Resende Bastos et al.
Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology], 49(3), 564-568 (2018-02-18)
The objective of this study was to standardize and validate the dot-blot test for the serological diagnosis of bovine brucellosis, compare the results with those found in the 2-mercaptoethanol (2-ME) and complement fixation test (CF), and estimate the relative sensitivity
Evaluation of an ELISA for detection of antibodies to Babesia bigemina in cattle and its application in an epidemiological survey in Brazil
Madruga, C., R., et al.
Pesquisa Veterinária Brasileira, 21(2), 72-76 (2001)
Detection of specific antibody responses to vaccination in variable flying foxes (Pteropus hypomelanus)
Wellehan Jr JFX, et al.
Comparative Immunology, Microbiology and Infectious Diseases, 32(5), 379-394 (2009)
Determination of lactoferrin and immunoglobulin G in animal milks by new immunosensors
Campanella L, et al.
Sensors, 9(3), 2202-2221 (2009)
B Gottstein et al.
International journal for parasitology, 28(4), 679-691 (1998-05-29)
Neospora caninum has gained considerable attention through its role in the aetiology of bovine abortion. Due to its close phylogenetic relationship with Toxoplasma gondii, respective unequivocal differential diagnosis deserves special consideration. In order to evaluate the diagnostic performance of molecular
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