51146
Atto 550 Protein Labeling Kit
BioReagent, suitable for fluorescence
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About This Item
UNSPSC Code:
12352200
NACRES:
NA.32
Recommended Products
product line
BioReagent
Quality Level
manufacturer/tradename
ATTO-TEC GmbH
fluorescence
λex 544 nm; λem 576 nm in 0.1 M phosphate buffer, pH 7.0 (recommended)
suitability
suitable for fluorescence
storage temp.
2-8°C
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General description
This kit contains sufficient amounts of reactive dye, buffers and protein purification sets for performing five labeling reactions (1 mg protein each) and for the subsequent purification of the labeled protein.
Application
Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation. Atto 550, which is similar to Cy3, may be useful in applications such as fluorescence resonance energy transfer (FRET) and as a tag for molecules such as secondary antibodies. Atto 550 Protein Labeling Kit contains Atto 550 succinimidyl ester which enables conjugation of Atto 550 to the primary amino groups of the proteins and other molecules.
Legal Information
This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 3
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Articles
Sigma® offers protein labeling kits based on two types of fluorescent dyes, the Atto dyes and the Tracy dyes.
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